大鼠开放性伤口实验模型中的炎症反应。花生四烯酸代谢产物的作用。
The inflammatory reaction in an experimental model of open wounds in the rat. The effect of arachidonic acid metabolites.
作者信息
Lundberg C, Gerdin B
出版信息
Eur J Pharmacol. 1984 Jan 27;97(3-4):229-38. doi: 10.1016/0014-2999(84)90454-0.
The study concerned the effect of arachidonic acid metabolites on the inflammatory reaction in granulation tissue of open wounds in rats. Metabolites or inhibitors were applied in a wound chamber attached to circular, open, full-thickness skin wounds 5 days post-wounding. The adjacent wound served as control. Blood flow, albumin extravasation and accumulation of polymorphonuclear leucocytes (PMNLs) were measured in the granulation tissue. Prostaglandin E2 (PGE2 5.7 microM) increased blood flow and albumin extravasation by 95 and 16%, respectively, without affecting PMNLs. Leukotriene B4 (LTB4 2.7 microM) increased PMNL accumulation by 142% without altering albumin extravasation. Indomethacin (28 microM, repeatedly) did not affect blood flow or albumin extravasation, but increased PMNL accumulation by 21%. Methylprednisolone (3.3 mM, repeatedly) reduced blood flow and albumin extravasation by 29 and 31%, respectively, without influencing PMNLs. The granulation tissue obviously responds to exogenous PGE2 and LTB4. Endogenous arachidonic acid metabolites seem to play only a minor role in the inflammatory process in this model.
该研究关注花生四烯酸代谢产物对大鼠开放性伤口肉芽组织炎症反应的影响。在创伤后5天，将代谢产物或抑制剂应用于附着在圆形、开放性、全层皮肤伤口上的伤口室中。相邻伤口作为对照。测量肉芽组织中的血流量、白蛋白外渗和多形核白细胞（PMNLs）的积聚情况。前列腺素E2（PGE2 5.7微摩尔）分别使血流量和白蛋白外渗增加了95%和16%，而不影响PMNLs。白三烯B4（LTB4 2.7微摩尔）使PMNL积聚增加了142%，而不改变白蛋白外渗。吲哚美辛（28微摩尔，重复使用）不影响血流量或白蛋白外渗，但使PMNL积聚增加了21%。甲基泼尼松龙（3.3毫摩尔，重复使用）分别使血流量和白蛋白外渗减少了29%和31%，而不影响PMNLs。肉芽组织明显对外源性PGE2和LTB4有反应。在该模型中，内源性花生四烯酸代谢产物在炎症过程中似乎只起次要作用。
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