Takada K, Zur Hausen H
Int J Cancer. 1984 Apr 15;33(4):491-6. doi: 10.1002/ijc.2910330412.
Fifteen established tumor promoters belonging to different chemical groups were tested for their ability to induce Epstein-Barr virus (EBV)-specific early antigens (EA) in EBV-genome-positive nonproducer Raji cells. Saccharin (a promoter in urinary bladder carcinogenesis), DDT (a promoter in liver carcinogenesis), anthralin and iodoacetic acid (promoters in skin carcinogenesis) gave a significant induction with a maximum of induced cells of 20% (8 mg/ml), 0.8% (20 micrograms/ml), 0.8% (100 ng/ml) and 0.7% (0.4 micrograms/ml), respectively. In addition, after combined application with a noninducing dose (0.2 ng/ml) of 12-O-tetradecanoyl-phorbol-13-acetate (TPA), seven additional tumor promoters induced 0.3-2.1% EA-positive cells two days after treatment. The results indicate that in addition to mouse skin tumor promoters such as diterpene esters, several compounds reported to possess tumor-promoting activity in other types of tissue induce EBV. The data suggest that EBV induction is an effect commonly exerted by this group of compounds which should be very useful in screening for environmental tumor promoters.
对15种属于不同化学组别的已确定的肿瘤促进剂进行了测试，以检测它们在EBV基因组阳性的非生产性Raji细胞中诱导爱泼斯坦-巴尔病毒（EBV）特异性早期抗原（EA）的能力。糖精（膀胱癌致癌中的一种促进剂）、滴滴涕（肝癌致癌中的一种促进剂）、蒽林和碘乙酸（皮肤癌致癌中的促进剂）均产生了显著诱导作用，诱导细胞的最大比例分别为20%（8毫克/毫升）、0.8%（20微克/毫升）、0.8%（100纳克/毫升）和0.7%（0.4微克/毫升）。此外，在与非诱导剂量（0.2纳克/毫升）的12-O-十四烷酰佛波醇-13-乙酸酯（TPA）联合应用后，另外七种肿瘤促进剂在处理两天后诱导出0.3 - 2.1%的EA阳性细胞。结果表明，除了二萜酯等小鼠皮肤肿瘤促进剂外，其他几种据报道在其他类型组织中具有肿瘤促进活性的化合物也能诱导EBV。数据表明，EBV诱导是这类化合物普遍产生的一种效应，这在筛选环境肿瘤促进剂方面应该非常有用。
