Chaintreuil J, Monnier L, Colette C, Crastes de Paulet P, Orsetti A, Spielmann D, Mendy F, Crastes de Paulet A
Hum Nutr Clin Nutr. 1984 Mar;38(2):121-30.
In order to gain insight into the mechanism of platelet dysfunction in insulin-dependent diabetics we studied in 17 patients the influence of a 6-week period with a dietary supplement of gamma-linolenate: 2 g/d (group I, n = 8) or 500 mg/d (group II, n = 9). Serum lipids, plasma beta-thromboglobulin (beta TG), platelet aggregation in vitro, and TxB2 and PGE1 released from platelets during the aggregation process were measured. In group I, serum triglycerides fell from 1.57 +/- 0.28 mmol/l to 0.99 +/- 0.17 mmol/l (P less than 0.01) and cholesterol fell from 5.85 +/- 0.55 mmol/l to 5.08 +/- 0.52 mmol/l (P less than 0.01). In group I plasma beta TG fell from 100.0 +/- 15.7 ng/ml to 73.7 +/- 12.1 ng/ml (P less than 0.025), while the sum of the percentages of the C18:3 omega 6 and its chain elongated (C20:3 omega 6) and desaturated (C20:4 omega 6) metabolites increased in serum triglycerides (P less than 0.05), cholesterol esters (P less than 0.02) and phospholipids (P less than 0.02). No changes were observed on the other parameters in either group. The results show that the lowering effects of gamma-linolenate on serum triglycerides, cholesterol and plasma beta TG occur only with daily intakes of 2 g. The changes in fatty acid composition of serum lipids suggest that the gamma-linolenate intake of 2 g may exert its beneficial effect through an increased incorporation of long-chain polyunsaturated fatty acids, but no firm conclusion can be drawn as membrane platelet fatty acid composition was not evaluated.
为深入了解胰岛素依赖型糖尿病患者血小板功能障碍的机制，我们对17例患者进行了研究，给予为期6周的γ-亚麻酸膳食补充剂：2g/天（第一组，n = 8）或500mg/天（第二组，n = 9）。测定了血清脂质、血浆β-血小板球蛋白（βTG）、体外血小板聚集以及聚集过程中血小板释放的TxB2和PGE1。在第一组中，血清甘油三酯从1.57±0.28mmol/l降至0.99±0.17mmol/l（P＜0.01），胆固醇从5.85±0.55mmol/l降至5.08±0.52mmol/l（P＜0.01）。在第一组中，血浆βTG从100.0±15.7ng/ml降至73.7±12.1ng/ml（P＜0.025），而血清甘油三酯（P＜0.05）、胆固醇酯（P＜0.02）和磷脂（P＜0.02）中C18:3ω6及其链延长（C20:3ω6）和去饱和（C20:4ω6）代谢产物的百分比总和增加。两组的其他参数均未观察到变化。结果表明，γ-亚麻酸对血清甘油三酯、胆固醇和血浆βTG的降低作用仅在每日摄入量为2g时出现。血清脂质脂肪酸组成的变化表明，摄入2gγ-亚麻酸可能通过增加长链多不饱和脂肪酸的掺入发挥有益作用，但由于未评估血小板膜脂肪酸组成，无法得出确切结论。
