Test S T, Weiss S J
J Biol Chem. 1984 Jan 10;259(1):399-405.
The extracellular H2O2 concentration surrounding stimulated human neutrophils was continuously quantitated with a sensitive, H2O2-detecting electrode. Following stimulation of neutrophils with phorbol myristate acetate, opsonized zymosan particles, or N-formyl-Met-Leu-Phe, the extracellular H2O2 concentration rapidly increased and maintained steady state conditions before falling to undetectable levels in a manner that was dependent on the triggering agent used. Total extracellular H2O2 accumulation for each stimulus was quantitated as the integral of the H2O2 concentration with respect to time. H2O2 accumulation in the extracellular milieu was unaffected by the addition of superoxide dismutase, whereas exogenous catalase or myeloperoxidase completely consumed the released H2O2. Analysis of H2O2 metabolism by neutrophils revealed that stimulus-dependent differences in the size of the extracellular H2O2 pool may be partially attributable to differences in hypochlorous acid generation by the H2O2, myeloperoxidase, chloride system. Finally, both the concentration of H2O2 in the extracellular space and its utilization by myeloperoxidase could be diminished in the presence of an extracellular target cell. These data indicate that the ability of a triggering agent to stimulate the neutrophil to generate H2O2 and release myeloperoxidase, coupled with the characteristics of a target cell population, control H2O2 metabolism in effector-target cell interactions.
使用灵敏的H2O2检测电极连续定量刺激后的人中性粒细胞周围的细胞外H2O2浓度。在用佛波酯肉豆蔻酸酯、调理酵母聚糖颗粒或N-甲酰甲硫氨酰亮氨酰苯丙氨酸刺激中性粒细胞后，细胞外H2O2浓度迅速升高并维持稳态，然后以依赖于所用触发剂的方式降至检测不到的水平。每种刺激的细胞外H2O2总积累量通过H2O2浓度相对于时间的积分来定量。超氧化物歧化酶的添加不影响细胞外环境中H2O2的积累，而外源性过氧化氢酶或髓过氧化物酶完全消耗释放的H2O2。对中性粒细胞H2O2代谢的分析表明，细胞外H2O2池大小的刺激依赖性差异可能部分归因于H2O2、髓过氧化物酶、氯离子系统产生次氯酸的差异。最后，在存在细胞外靶细胞的情况下，细胞外空间中H2O2的浓度及其被髓过氧化物酶的利用都可能降低。这些数据表明，触发剂刺激中性粒细胞产生H2O2和释放髓过氧化物酶的能力，以及靶细胞群体的特性，在效应器-靶细胞相互作用中控制H2O2代谢。
