Nedrud J G, Wu R
J Gen Virol. 1984 Apr;65 ( Pt 4):671-9. doi: 10.1099/0022-1317-65-4-671.
Recently, methods have been developed to culture dissociated tracheal epithelial (TE) cells. Attempts were made to infect these epithelial cells with mouse cytomegalovirus (MCMV) to see if the dissociated epithelial cells share characteristics of infection with MCMV-infected tracheal organ cultures. When isolated TE cells were incubated with MCMV at multiplicities between 0.10 and 2.0, no infection or minimal infection resulted. Centrifugation of virus onto the TE cell sheets also resulted in only minimal infection. If MCMV-infected mouse embryo fibroblasts were added to cultures of TE cells, however, the TE cells subsequently became infected. TE cells could also be infected by incubating MCMV with mixed cultures of uninfected fibroblasts and TE cells. The epithelial nature of infected cells was confirmed by electron microscopy. Reconstruction experiments demonstrated that fibroblast-mediated infection of mouse TE cells with MCMV was not simply due to the large amounts of virus provided by the infected fibroblasts. It is suggested that cell-cell contact, or fusion with infected cells is required for productive infection of TE cells with MCMV.
最近，已开发出培养解离的气管上皮（TE）细胞的方法。人们尝试用小鼠巨细胞病毒（MCMV）感染这些上皮细胞，以观察解离的上皮细胞是否具有与MCMV感染的气管器官培养物相同的感染特征。当分离的TE细胞与MCMV以0.10至2.0的感染复数进行孵育时，未产生感染或仅有极少的感染发生。将病毒离心到TE细胞片上也仅导致极少的感染。然而，如果将感染MCMV的小鼠胚胎成纤维细胞添加到TE细胞培养物中，随后TE细胞就会被感染。通过将MCMV与未感染的成纤维细胞和TE细胞的混合培养物一起孵育，TE细胞也可被感染。通过电子显微镜证实了感染细胞的上皮性质。重建实验表明，成纤维细胞介导的MCMV对小鼠TE细胞的感染并非仅仅是由于感染的成纤维细胞提供了大量病毒。提示TE细胞被MCMV有效感染需要细胞间接触或与感染细胞融合。
