Harnett G B, Shellam G R
J Gen Virol. 1982 Sep;62 (Pt 1):39-47. doi: 10.1099/0022-1317-62-1-39.
The in vitro growth of murine cytomegalovirus (MCMV) in mouse embryo fibroblasts (MEF) from Balb/c, C57BL, C3H and CBA mouse strains has been shown to correlate with the genetically determined in vivo susceptibility of these strains to MCMV infection. MEF from Balb/c and C57BL mice were more susceptible to MCMV than those from C3H and CBA mice. This was evident regardless of whether replication was measured by cytopathic effect (c.p.e.) score, virus yield, plaque count, plaque size or time of onset of c.p.e. The growth of MCMV in tracheal organ cultures from different mouse strains was similar to that observed in MEF from these strains. The replication in MEF when measured by c.p.e. score and virus yield was affected by the density of the cell cultures. The strain of mouse used to produce MCMV also affected the comparative sensitivity of MEF to the virus. This appeared to be due to reduced growth of MCMV in its homologous MEF type, an unexpected result. In contrast, cell density had little effect on the replication of herpes simplex virus type 1 (HSV-1), but again the in vitro susceptibility of MEF reflected the in vivo susceptibility of mouse strains to infection with this virus.
已证明小鼠巨细胞病毒（MCMV）在来自Balb/c、C57BL、C3H和CBA小鼠品系的小鼠胚胎成纤维细胞（MEF）中的体外生长与这些品系在遗传上决定的对MCMV感染的体内易感性相关。来自Balb/c和C57BL小鼠的MEF比来自C3H和CBA小鼠的MEF对MCMV更敏感。无论通过细胞病变效应（c.p.e.）评分、病毒产量、蚀斑计数、蚀斑大小还是c.p.e.出现时间来衡量复制情况，这一点都很明显。MCMV在来自不同小鼠品系的气管器官培养物中的生长情况与在这些品系的MEF中观察到的情况相似。通过c.p.e.评分和病毒产量来衡量时，MEF中的复制受细胞培养物密度的影响。用于产生MCMV的小鼠品系也影响MEF对该病毒的比较敏感性。这似乎是由于MCMV在其同源MEF类型中的生长减少，这是一个意想不到的结果。相比之下，细胞密度对1型单纯疱疹病毒（HSV-1）的复制影响很小，但同样，MEF的体外易感性反映了小鼠品系对该病毒感染的体内易感性。
