用于放射免疫分析的新型甾体半抗原的合成。第四部分。雌激素的3 - O - 羧甲基醚衍生物。雌酮、雌二醇 - 17β和雌三醇放射免疫分析的特异性抗血清。
Synthesis of new steroid haptens for radioimmunossay. Part IV. 3-O-Carboxymethyl ether derivatives of estrogens. Specific antisera for radioimmunossay of estrone, estradiol-17beta, and estriol.
作者信息
Rao P N, Moore P H
出版信息
Steroids. 1977 Apr;29(4):461-9. doi: 10.1016/0039-128x(77)90066-6.
PMID:867445
Abstract
The syntheses of 3-O-carboxymethyl ether derivatives of estrone, estradiol-17beta, and estriol and the preparation of their bovine serum albumin (BSA) conjugates are described. These conjugates were employed for the generation of specific antisera suitable for radioimmunoassay (RIA) of estrone, estradiol-17beta, and estriol. The previous concept that specific antisera for estrogens cannot be obtained by employing estrogens derivatized at the 3-position is unfounded.
摘要
描述了雌酮、17β-雌二醇和雌三醇的3-O-羧甲基醚衍生物的合成及其牛血清白蛋白（BSA）缀合物的制备。这些缀合物用于产生适用于雌酮、17β-雌二醇和雌三醇放射免疫测定（RIA）的特异性抗血清。以前认为不能通过使用在3位衍生化的雌激素获得雌激素特异性抗血清的概念是没有根据的。
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