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海胆H2A基因调节剂的一个功能组件包含一段与病毒增强子的延伸序列同源性。

A functional component of the sea urchin H2A gene modulator contains an extended sequence homology to a viral enhancer.

作者信息

Grosschedl R, Mächler M, Rohrer U, Birnstiel M L

出版信息

Nucleic Acids Res. 1983 Dec 10;11(23):8123-36. doi: 10.1093/nar/11.23.8123.

DOI:10.1093/nar/11.23.8123
PMID:6324073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326570/
Abstract

The DNA sequences imparting a maximal rate of sea urchin H2A gene transcription in the frog oocyte nucleus were narrowed down by deletion mapping to a DNA segment -165 to -111, far-upstream of the H2A mRNA cap site. C to T base changes in this area create strong down mutations, hence the primary structure of this DNA sequence is of paramount importance to the H2A gene expression. Sequence comparisons suggest that the -165 to -111 region may contain two essential sequence blocks. Most strikingly, the -135 area contains a 14 out of 17 basepair homology to the Moloney murine sarcoma virus enhancer and to topologically related 5' LTR-sequences of the simian sarcoma virus and the murine Friend spleen focus forming virus.

摘要

通过缺失定位，确定了在蛙卵母细胞核中赋予海胆H2A基因最大转录速率的DNA序列，该序列位于H2A mRNA帽位点上游很远的-165至-111的DNA片段。该区域的C到T碱基变化会产生强烈的负向突变，因此该DNA序列的一级结构对H2A基因表达至关重要。序列比较表明，-165至-111区域可能包含两个必需的序列块。最引人注目的是，-135区域与莫洛尼鼠肉瘤病毒增强子以及猿猴肉瘤病毒和鼠类Friend脾集落形成病毒的拓扑相关5' LTR序列有17个碱基对中的14个碱基对的同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea96/326570/f22d212dd2d9/nar00368-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea96/326570/24d68edab0a7/nar00368-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea96/326570/f22d212dd2d9/nar00368-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea96/326570/24d68edab0a7/nar00368-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea96/326570/f22d212dd2d9/nar00368-0022-a.jpg

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本文引用的文献

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EMBO J. 1983;2(8):1423-9. doi: 10.1002/j.1460-2075.1983.tb01601.x.
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Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.一个编码组蛋白变体的海胆组蛋白DNA克隆中普遍存在的和基因特异性的调控5'序列。
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Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
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Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6854-8. doi: 10.1073/pnas.90.14.6854.
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