• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

海胆早期组蛋白基因在胚胎发生过程中染色质结构的瞬时改变。

Transient alterations of the chromatin structure of sea urchin early histone genes during embryogenesis.

作者信息

Wu T C, Simpson R T

出版信息

Nucleic Acids Res. 1985 Sep 11;13(17):6185-203. doi: 10.1093/nar/13.17.6185.

DOI:10.1093/nar/13.17.6185
PMID:2995919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321946/
Abstract

We describe features of the chromatin structure of the early histone gene family of Strongylocentrotus purpuratus during development. Before and after the histone genes are transcriptionally active, chromatin structure is quite similar with well-defined spaced nucleosomes and no major 5'-flanking sites hypersensitive to nucleases. During the period when the genes are active, marked changes in chromatin structure occur. Micrococcal nuclease digestion generates monomer nucleosomes and only trace amounts of higher multimers. Regions hypersensitive to an endogenous nuclease and DNAase I appear in the 5'-flanking regions of genes for H2A, H2B and H3. Each region consists of four sites spanning a DNA length of 200-250 base pairs. In each case, one major cutting site is near the TATA box; the bulk of the sensitive region is in the nontranscribed spacer. Other sites, in 3'-flanking regions of the genes, are sensitive to nucleases only when the histone genes are no longer transcribed.

摘要

我们描述了紫海胆早期组蛋白基因家族在发育过程中的染色质结构特征。在组蛋白基因转录激活之前和之后，染色质结构非常相似，有明确间隔的核小体，且没有对核酸酶高度敏感的主要5'侧翼位点。在基因活跃的时期，染色质结构发生了显著变化。微球菌核酸酶消化产生单体核小体，只有微量的更高多聚体。对内源核酸酶和DNA酶I高度敏感的区域出现在H2A、H2B和H3基因的5'侧翼区域。每个区域由跨越200 - 250个碱基对DNA长度的四个位点组成。在每种情况下，一个主要切割位点靠近TATA框；大部分敏感区域位于非转录间隔区。基因3'侧翼区域的其他位点仅在组蛋白基因不再转录时对核酸酶敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/fd9bb51cddfb/nar00311-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/bc8e1c08156d/nar00311-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/569b070b5839/nar00311-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/1f7ddc09a535/nar00311-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/3946ca7a410b/nar00311-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/fd9bb51cddfb/nar00311-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/bc8e1c08156d/nar00311-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/569b070b5839/nar00311-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/1f7ddc09a535/nar00311-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/3946ca7a410b/nar00311-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ad/321946/fd9bb51cddfb/nar00311-0185-a.jpg

相似文献

1
Transient alterations of the chromatin structure of sea urchin early histone genes during embryogenesis.海胆早期组蛋白基因在胚胎发生过程中染色质结构的瞬时改变。
Nucleic Acids Res. 1985 Sep 11;13(17):6185-203. doi: 10.1093/nar/13.17.6185.
2
Chromatin structure of the developmentally regulated early histone genes of the sea urchin Strongylocentrotus purpuratus.紫海胆发育调控早期组蛋白基因的染色质结构。
Nucleic Acids Res. 1990 Sep 11;18(17):5255-63. doi: 10.1093/nar/18.17.5255.
3
Site and stage specific action of endogenous nuclease and micrococcal nuclease on histone genes of sea urchin embryos.内源性核酸酶和微球菌核酸酶对海胆胚胎组蛋白基因的位点和阶段特异性作用。
Dev Biol. 1986 Sep;117(1):109-13. doi: 10.1016/0012-1606(86)90353-2.
4
Chromatin structure of histone genes in sea urchin sperms and embryos.海胆精子和胚胎中组蛋白基因的染色质结构。
Nucleic Acids Res. 1982 Dec 20;10(24):7977-91. doi: 10.1093/nar/10.24.7977.
5
Different micrococcal nuclease cleavage patterns characterize transcriptionally active and inactive sea-urchin histone genes.
Eur J Biochem. 1986 Apr 15;156(2):367-74. doi: 10.1111/j.1432-1033.1986.tb09592.x.
6
Major changes in the 5' and 3' chromatin structure of sea urchin histone genes accompany their activation and inactivation in development.海胆组蛋白基因5'和3'染色质结构的重大变化伴随着它们在发育过程中的激活和失活。
Cell. 1983 Jul;33(3):843-8. doi: 10.1016/0092-8674(83)90026-0.
7
Developmentally-regulated interaction of a transcription factor complex containing CDP/cut with the early histone H3 gene promoter of the sea urchin Tetrapygus niger is associated with changes in chromatin structure and gene expression.包含CDP/cut的转录因子复合物与海胆四疣黑星海胆早期组蛋白H3基因启动子的发育调控相互作用与染色质结构和基因表达的变化相关。
Gene. 2001 Jul 11;272(1-2):237-48. doi: 10.1016/s0378-1119(01)00534-0.
8
Distribution of histone variants in the sea urchin chromatin fractions obtained by selective micrococcal nuclease digestion.通过选择性微球菌核酸酶消化获得的海胆染色质组分中组蛋白变体的分布。
Mol Biol Rep. 1985 Oct;10(4):199-203. doi: 10.1007/BF00775976.
9
Histone variants and chromatin structure during sea urchin development.海胆发育过程中的组蛋白变体与染色质结构
Dev Biol. 1980 Nov;80(1):186-209. doi: 10.1016/0012-1606(80)90508-4.
10
Modulation of nucleosome structure by histone subtypes in sea urchin embryos.海胆胚胎中组蛋白亚型对核小体结构的调控。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6803-7. doi: 10.1073/pnas.78.11.6803.

引用本文的文献

1
Dynamics of interaction of RNA polymerase II with nucleosomes. II. During read-through and elongation.RNA聚合酶II与核小体相互作用的动力学。II.通读和延伸过程中。
Protein Sci. 1993 Dec;2(12):2246-58. doi: 10.1002/pro.5560021224.
2
Purification of an oligo(dG).oligo(dC)-binding sea urchin nuclear protein, suGF1: a family of G-string factors involved in gene regulation during development.一种寡聚（dG）.寡聚（dC）结合海胆核蛋白suGF1的纯化：参与发育过程中基因调控的G串因子家族。
Mol Cell Biol. 1994 Feb;14(2):1402-9. doi: 10.1128/mcb.14.2.1402-1409.1994.
3
Chromatin transitions during activation and repression of galactose-regulated genes in yeast.

本文引用的文献

1
The rate of synthesis of histone mRNA during the development of sea urchin embryos (Strongylocentrotus purpuratus).海胆胚胎（紫球海胆）发育过程中组蛋白mRNA的合成速率。
Dev Biol. 1981 Apr 30;83(2):380-6. doi: 10.1016/0012-1606(81)90485-1.
2
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.T-A-T-A-A-A-T-A序列上游的间隔DNA序列对于体内H2A组蛋白基因转录的促进至关重要。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6. doi: 10.1073/pnas.77.12.7102.
3
Major changes in the 5' and 3' chromatin structure of sea urchin histone genes accompany their activation and inactivation in development.
酵母中半乳糖调节基因激活和抑制过程中的染色质转变。
EMBO J. 1993 Dec;12(12):4603-13. doi: 10.1002/j.1460-2075.1993.tb06149.x.
4
Formation of stable chromatin structures on the histone H4 gene during differentiation in Tetrahymena thermophila.嗜热四膜虫分化过程中组蛋白H4基因上稳定染色质结构的形成。
Mol Cell Biol. 1986 Aug;6(8):3014-7. doi: 10.1128/mcb.6.8.3014-3017.1986.
5
Chromosomal organization of the herpes simplex virus genome during acute infection of the mouse central nervous system.单纯疱疹病毒基因组在小鼠中枢神经系统急性感染期间的染色体组织。
J Virol. 1986 Sep;59(3):764-7. doi: 10.1128/JVI.59.3.764-767.1986.
6
Cis-acting elements of the sea urchin histone H2A modulator bind transcriptional factors.海胆组蛋白H2A调节剂的顺式作用元件结合转录因子。
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6033-7. doi: 10.1073/pnas.86.16.6033.
7
Chromatin structure of the developmentally regulated early histone genes of the sea urchin Strongylocentrotus purpuratus.紫海胆发育调控早期组蛋白基因的染色质结构。
Nucleic Acids Res. 1990 Sep 11;18(17):5255-63. doi: 10.1093/nar/18.17.5255.
8
Active beta-globin gene transcription occurs in methylated, DNase I-resistant chromatin of nonerythroid chicken cells.活性β-珠蛋白基因转录发生在非红细胞系鸡细胞的甲基化、对DNA酶I有抗性的染色质中。
Mol Cell Biol. 1990 Jan;10(1):16-27. doi: 10.1128/mcb.10.1.16-27.1990.
9
Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.核蛋白杂交：一种将活性和非活性基因作为染色质进行分离的方法。
Nucleic Acids Res. 1991 Mar 25;19(6):1325-36. doi: 10.1093/nar/19.6.1325.
海胆组蛋白基因5'和3'染色质结构的重大变化伴随着它们在发育过程中的激活和失活。
Cell. 1983 Jul;33(3):843-8. doi: 10.1016/0092-8674(83)90026-0.
4
Base sequence and helix structure variation in B and A DNA.B型和A型DNA的碱基序列及螺旋结构变异
J Mol Biol. 1983 May 25;166(3):419-41. doi: 10.1016/s0022-2836(83)80093-x.
5
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
Nature. 1980 Aug 28;286(5776):854-60. doi: 10.1038/286854a0.
6
Chromatin structure and gene activity: the role of nonhistone chromosomal proteins.染色质结构与基因活性：非组蛋白染色体蛋白的作用
CRC Crit Rev Biochem. 1982;13(1):1-86. doi: 10.3109/10409238209108709.
7
Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region.热休克激活的hsp 70基因的染色质结构：组蛋白从编码区的选择性去除及其在5'区域的缺失。
Cell. 1984 Feb;36(2):423-31. doi: 10.1016/0092-8674(84)90235-6.
8
Nuclease digestion of circular TRP1ARS1 chromatin reveals positioned nucleosomes separated by nuclease-sensitive regions.环状TRP1ARS1染色质的核酸酶消化显示，定位核小体被核酸酶敏感区域隔开。
J Mol Biol. 1984 Aug 25;177(4):715-33. doi: 10.1016/0022-2836(84)90046-9.
9
A functional component of the sea urchin H2A gene modulator contains an extended sequence homology to a viral enhancer.海胆H2A基因调节剂的一个功能组件包含一段与病毒增强子的延伸序列同源性。
Nucleic Acids Res. 1983 Dec 10;11(23):8123-36. doi: 10.1093/nar/11.23.8123.
10
Promoter elements, regulatory elements, and chromatin structure of the yeast his3 gene.酵母组氨酸3基因的启动子元件、调控元件及染色质结构
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:901-10. doi: 10.1101/sqb.1983.047.01.104.