Yam L T, Li C Y
Am J Clin Pathol. 1976 Dec;66(6):976-85. doi: 10.1093/ajcp/66.6.976.
Histochemical markers were used to identify the various cellular and structural components of the human spleen, and to investigate the histogenesis of the splenic lesions of Hodgkin's disease. The early lesions appear in areas near the central artery (periarterial lymphatic sheath) in the white pulp. The white pulp becomes hypertrophic. The lesions enlarge, extend into the red pulp, and compress the sinuses and the cords of Billroth. The derivations of various "histiocytes" contained with the lesions are differentiated by using cytochemical stains for lysosomal enzymes and for granulocytes. The epithelioid cells in the granulomas are rich in those lysosomal enzymes typically seen in phagocytic histiocytes, suggesting that they are indeed true histiocytes. The malignant "histiocytes," including the mononuclear Hodgkin cells, the binucleated Sternberg-Reed cells, and the multinucleated giant cells, do not contain significant amounts of lysosomal enzymes and more closely resemble stimulated lymphocytes. The splenic lesions in Hodkin's disease may be the result of a lymphocytic and histiocytic cellular response to an unknown agent, which reaches the spleen through the central artery in the white pulp.
组织化学标记物用于识别人脾脏的各种细胞和结构成分，并研究霍奇金病脾脏病变的组织发生。早期病变出现在白髓中央动脉附近（动脉周围淋巴鞘）的区域。白髓变得肥大。病变扩大，延伸至红髓，并压迫血窦和脾索。通过使用针对溶酶体酶和粒细胞的细胞化学染色来区分病变中所含各种“组织细胞”的来源。肉芽肿中的上皮样细胞富含吞噬性组织细胞中常见的那些溶酶体酶，这表明它们确实是真正的组织细胞。恶性“组织细胞”，包括单核霍奇金细胞、双核施特恩伯格-里德细胞和多核巨细胞，不含大量溶酶体酶，更类似于活化的淋巴细胞。霍奇金病的脾脏病变可能是淋巴细胞和组织细胞对未知因子的细胞反应的结果，该未知因子通过白髓中的中央动脉到达脾脏。
