山羊支原体核糖体RNA基因的物理图谱
Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum.
作者信息
Glaser G, Amikam D, Razin S
出版信息
Nucleic Acids Res. 1984 Mar 12;12(5):2421-6. doi: 10.1093/nar/12.5.2421.
Abstract
Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and BglII and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5'-16S-23S-5S-3', resembling the order of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.
摘要
通过用EcoRI、PstI和BglII消化山羊支原体的DNA，并与由大肠杆菌rrnB核糖体RNA操纵子的特定部分组成的缺口平移探针杂交，对山羊支原体的rRNA基因进行了物理图谱分析。结果表明，山羊支原体染色体中的rRNA基因排列成两个簇，每个簇按5'-16S-23S-5S-3'的顺序组织，类似于rrnB操纵子中的基因顺序，每个簇中的基因之间没有大的间隔区。
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