Glaser G, Razin A, Razin S
Nucleic Acids Res. 1981 Aug 11;9(15):3641-6. doi: 10.1093/nar/9.15.3641.
The synthesis of stable RNA in Mycoplasma capricolum was studied by [32P] labeling of cellular RNA of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. The results indicate that M. capricolum employs the same stringent control mechanism used by E. coli cells, as judged by a decreased synthesis of stable RNA and accumulation of 5'-triphosphoguanosine-3'-diphosphate (pppGpp) and 5'-diphosphoguanosine-3'-diphosphate (ppGpp) in response to amino acid starvation. In addition, the results suggest that precursors of stable RNA accumulate and an intracellular pool of the precursors exists at all times under the growth conditions used by us. These findings may be interpreted to reflect a slow rate of RNA processing in M. capricolum.
通过在部分限定培养基中生长的细胞的细胞RNA的[32P]标记，研究了山羊支原体中稳定RNA的合成，该培养基中存在或不存在氨基酸混合物补充剂。结果表明，根据稳定RNA合成减少以及响应氨基酸饥饿时5'-三磷酸鸟苷-3'-二磷酸（pppGpp）和5'-二磷酸鸟苷-3'-二磷酸（ppGpp）的积累来判断，山羊支原体采用了与大肠杆菌细胞相同的严谨控制机制。此外，结果表明稳定RNA的前体积累，并且在我们使用的生长条件下，前体的细胞内池始终存在。这些发现可以解释为反映了山羊支原体中RNA加工的缓慢速率。
