Chen X, Finch L R
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
J Bacteriol. 1989 May;171(5):2876-8. doi: 10.1128/jb.171.5.2876-2878.1989.
Large restriction fragments from the DNA of Mycoplasma gallisepticum S6 and PG31, which were prepared by digestion with BglI, BssHII, SmaI, or XhoI and which were separated by pulsed-field electrophoresis, were hybridized with probes containing most, or different parts, of an rRNA operon of Mycoplasma capricolum. The results showed that the genomes contained three widely separated rRNA loci. One locus contained genes for all three rRNA species and another contained 23S and probably 5S rRNA genes, whereas the third appeared to have only a 16S rRNA gene.
用BglI、BssHII、SmaI或XhoI消化制备的鸡毒支原体S6和PG31 DNA的大片段限制性片段，经脉冲场电泳分离后，与含有山羊支原体rRNA操纵子大部分或不同部分的探针杂交。结果表明，基因组包含三个广泛分离的rRNA基因座。一个基因座包含所有三种rRNA种类的基因，另一个包含23S和可能的5S rRNA基因，而第三个似乎只有一个16S rRNA基因。
