Payment P, Fortin S, Trudel M
Appl Environ Microbiol. 1984 Mar;47(3):591-2. doi: 10.1128/aem.47.3.591-592.1984.
The addition of 2.5 mM ferric chloride to 0.5% beef extract solution at pH 3.5 was found to be highly efficient in the recovery of seeded poliovirus type 1 (Sabin) or indigenous viruses from environmental samples. This method was extremely useful to reconcentrate viruses from beef extract solutions that did not flocculate at pH 3.5.
发现在pH 3.5的0.5%牛肉膏溶液中添加2.5 mM氯化铁,对于从环境样本中回收接种的1型脊髓灰质炎病毒(萨宾株)或本土病毒非常有效。该方法对于从在pH 3.5时未絮凝的牛肉膏溶液中重新浓缩病毒极为有用。