细胞内注射环磷酸腺苷（cAMP）对哺乳动物心脏细胞电偶联的影响。

Effect of intracellular injection of cAMP on the electrical coupling of mammalian cardiac cells.

作者信息

De Mello W C

出版信息

Biochem Biophys Res Commun. 1984 Mar 30;119(3):1001-7. doi: 10.1016/0006-291x(84)90873-8.

Abstract

The influence of cAMP on the electrical coupling of canine Purkinje fibers was investigated. It was found that the intracellular injection of the nucleotide enhances the cell-to-cell coupling appreciably. No change in the coupling coefficient (V2/V1) was found with the intracellular injection of 5-AMP. A slight decrease in input resistance (Vo/Io) was produced by cAMP injection and the time constant of the cell membrane (tau m) was also reduced. These findings indicate that the changes in intercellular coupling produced by cAMP were not related to an increase in resistance of the non-junctional membrane but to a decline in junctional resistance. The present results support the view that cAMP plays an important role in the modulation of junctional conductance in cardiac fibers.

摘要

研究了环磷酸腺苷（cAMP）对犬浦肯野纤维电偶联的影响。发现向细胞内注射该核苷酸可显著增强细胞间偶联。向细胞内注射5 - 腺苷酸（5 - AMP）时，偶联系数（V2/V1）未发生变化。注射cAMP导致输入电阻（Vo/Io）略有降低，细胞膜时间常数（tau m）也减小。这些发现表明，cAMP引起的细胞间偶联变化与非连接膜电阻增加无关，而是与连接电阻降低有关。目前的结果支持这样一种观点，即cAMP在调节心脏纤维连接电导中起重要作用。