细胞内注射钙和锶对心脏细胞通讯的影响。
Effect of intracellular injection of calcium and strontium on cell communication in heart.
作者信息
De Mello W C
出版信息
J Physiol. 1975 Sep;250(2):231-45. doi: 10.1113/jphysiol.1975.sp011051.
Abstract
- The influence of Ca and Sr on the electrical coupling of canine Purkinje cells was investigated by injecting the ions electrophoretically into the cytoplasm. 2. It was found that the intracellular injection produced electrical uncoupling which was spontaneously reversed. 3. No change in resting potential of the cell adjacent to the injection site was found except in those fibres not completely healed. 4. The input resistance of the injected cell increased concomitantly with the establishment of the electrical uncoupling. 5. Caffeine (6mM), added to the extracellular fluid, reduced the rate of spontaneous recoupling. Reduction of temperature of the Tyrode solution had the same effect. 6. The abolition of cell communication produced by Ca injection seems to indicate that the ion plays an important role in the control of junctional conductance in heart fibres.
摘要
- 通过电泳将钙和锶离子注入犬浦肯野细胞的细胞质，研究了它们对细胞电偶联的影响。2. 发现细胞内注射会产生电去偶联，且这种去偶联会自发逆转。3. 除了那些未完全愈合的纤维外，未发现注射部位相邻细胞的静息电位有变化。4. 随着电去偶联的建立，注射细胞的输入电阻随之增加。5. 向细胞外液中添加6毫摩尔咖啡因可降低自发再偶联的速率。降低台氏液的温度也有同样的效果。6. 钙注射导致的细胞通讯阻断似乎表明，该离子在心脏纤维连接电导的控制中起重要作用。
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