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链霉菌无细胞提取物对脯氨酸的羟基化作用。

Proline hydroxylation by cell free extract of a streptomycete.

作者信息

Onishi M, Okumura Y, Okamoto R, Ishikura T

出版信息

Biochem Biophys Res Commun. 1984 Apr 16;120(1):45-51. doi: 10.1016/0006-291x(84)91411-6.

DOI:10.1016/0006-291x(84)91411-6
PMID:6324794
Abstract

Free L-proline was hydroxylated to free L-hydroxyproline by cell free extract of Streptomyces griseoviridus P8648. The hydroxylation reaction required ferrous ion, 2-ketoglutarate and ascorbate. Zinc ion, ethylenediaminetetraacetic acid and alpha,alpha'-dipyridyl inhibited the reaction. Optimum temperature and pH were 25.0 degrees C and 7.5, respectively.

摘要

灰色链霉菌P8648的无细胞提取物可将游离L-脯氨酸羟基化为游离L-羟脯氨酸。羟基化反应需要亚铁离子、2-酮戊二酸和抗坏血酸。锌离子、乙二胺四乙酸和α,α'-联吡啶可抑制该反应。最适温度和pH分别为25.0℃和7.5。

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