包含大肠杆菌起始因子IF3结构基因的1.26千碱基DNA片段的序列：存在AUU起始密码子。
Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.
作者信息
Sacerdot C, Fayat G, Dessen P, Springer M, Plumbridge J A, Grunberg-Manago M, Blanquet S
出版信息
EMBO J. 1982;1(3):311-5. doi: 10.1002/j.1460-2075.1982.tb01166.x.
Abstract
The nucleotide sequence of a 1.26-kb pair DNA fragment containing the structural gene for Escherichia coli initiation factor IF3 has been determined. An open reading frame of 540 nucleotides is found at the position predicted by genetic studies. The amino-acid sequence deduced from the DNA sequence accounts for a molecular weight 20 530. The important feature of the coding DNA sequence is the presence of AUU as the translational initiator codon. It is 11 bases downstream of the center of a GGAGG sequence, which can strongly pair with the sequence CCUCC near the 3' terminus of 16S rRNA. The primary DNA sequence in the region of the AUU initiator codon and its role in compensating a reduced codon-anticodon interaction in initiation complex formation are discussed.
摘要
已确定了一段包含大肠杆菌起始因子IF3结构基因的1.26千碱基对DNA片段的核苷酸序列。在遗传学研究预测的位置发现了一个540个核苷酸的开放阅读框。从DNA序列推导的氨基酸序列的分子量为20530。编码DNA序列的重要特征是存在AUU作为翻译起始密码子。它位于GGAGG序列中心下游11个碱基处，该序列可与16S rRNA 3'末端附近的CCUCC序列强烈配对。讨论了AUU起始密码子区域的主要DNA序列及其在起始复合物形成中补偿减少的密码子-反密码子相互作用的作用。
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