大肠杆菌温度敏感型gyrB突变体中的基因表达
Gene expression in a temperature-sensitive gyrB mutant of Escherichia coli.
作者信息
Wahle E, Mueller K, Orr E
出版信息
EMBO J. 1984 Feb;3(2):315-20. doi: 10.1002/j.1460-2075.1984.tb01803.x.
Abstract
The effect of gyrase inactivation on gene expression was studied by examining the activities of different promoters in a temperature-sensitive gyrB mutant of Escherichia coli. The relative activities of promoters affected by cAMP-binding protein (CAP), e.g., the lac promoter, are not reduced by gyrase inactivation but can, on the contrary, be enhanced. This stimulation depends on the promoter location or its structure. The tnaA promoter is activated when located near the origin of replication, suggesting a differential effect of gyrase inactivation on various chromosomal domains. Only silent or mutant promoters such as the non-functional wild-type bgl or the lacIq can be activated. No differential effect of gyrase inactivation on the lambda pL and the trp promoters carried by the phage can be detected.
摘要
通过检测大肠杆菌温度敏感型gyrB突变体中不同启动子的活性，研究了gyrase失活对基因表达的影响。受环磷酸腺苷结合蛋白（CAP）影响的启动子，如乳糖启动子的相对活性，不会因gyrase失活而降低，相反，可能会增强。这种刺激取决于启动子的位置或其结构。当tnaA启动子位于复制起点附近时会被激活，这表明gyrase失活对不同染色体结构域有不同的影响。只有沉默或突变的启动子，如无功能的野生型bgl或lacIq才能被激活。未检测到gyrase失活对噬菌体携带的λ pL和色氨酸启动子有不同影响。
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