DNA 回旋酶失活对大肠杆菌 RNA 合成的影响。
Effect of DNA gyrase inactivation on RNA synthesis in Escherichia coli.
作者信息
Wahle E, Mueller K, Orr E
出版信息
J Bacteriol. 1985 Apr;162(1):458-60. doi: 10.1128/jb.162.1.458-460.1985.
Abstract
The average chain growth rates of rRNA and of total RNA were not affected by a thermal inactivation of DNA gyrase in a temperature-sensitive gyrB mutant of Escherichia coli. The fact that total RNA synthesis decreased under these conditions suggests that transcription is primarily affected at the step of chain initiation. The fraction of rRNA in total pulse-labeled RNA was not altered by inactivation of the enzyme, indicating that the latter is not required to actively maintain a high rate of synthesis of this RNA species.
摘要
在大肠杆菌温度敏感型gyrB突变体中，DNA促旋酶的热失活并未影响rRNA和总RNA的平均链生长速率。在这些条件下总RNA合成减少这一事实表明，转录主要在链起始步骤受到影响。酶失活并未改变脉冲标记的总RNA中rRNA的比例，这表明并不需要后者来积极维持这种RNA种类的高合成速率。
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