Mandecki W, Reznikoff W S
Nucleic Acids Res. 1982 Feb 11;10(3):903-12. doi: 10.1093/nar/10.3.903.
A lac promotor mutant was constructed by filling in the protruding ends of the HpaII site located within the lac promotor. The mutation, named M42, is a two base pair insertion that changes the distance between the -10 and -35 regions from 18 to 20 residues. The activity of the mutant promotor measured in vivo is 15% of the wild-type promotor. The M42 promotor is sensitive to the catabolite repression in the manner similar to that of the wild type. Sequences of several deletions within the lac promotor are also given.
通过填补位于乳糖启动子内的HpaII位点的突出末端构建了一个乳糖启动子突变体。该突变体名为M42，是一个两碱基对的插入，它将-10和-35区域之间的距离从18个残基改变为20个残基。在体内测量的突变启动子的活性是野生型启动子的15%。M42启动子以与野生型类似的方式对分解代谢物阻遏敏感。还给出了乳糖启动子内几个缺失的序列。
