通过使用区域实验室的转运器增强病毒分离。
Enhanced virus isolation by use of the transporter for a regional laboratory.
作者信息
Warford A L, Eveland W G, Strong C A, Levy R A, Rekrut K A
出版信息
J Clin Microbiol. 1984 Apr;19(4):561-2. doi: 10.1128/jcm.19.4.561-562.1984.
Abstract
Transporter tubes with fibroblast monolayers were compared with sucrose-phosphate-glutamate buffer for transport of 385 specimens to a regional laboratory for virus culture. Ninety-two (91%) of 101 viral isolates were recovered in cell culture from the Transporter, as compared with 82 (81%) from sucrose-phosphate-glutamate buffer. Twenty-five (24.7%) of the viral isolates were detected by cytopathic effect from 1 to 11 days earlier from the Transporter than from the sucrose-phosphate-glutamate buffer. The Transporter also permitted the use of ambient temperature for transport.
摘要
将含有成纤维细胞单层的转运管与蔗糖 - 磷酸盐 - 谷氨酸缓冲液进行比较，用于将385份标本运送到地区实验室进行病毒培养。从转运管中进行细胞培养时，101株病毒分离株中有92株（91%）被回收，而从蔗糖 - 磷酸盐 - 谷氨酸缓冲液中回收的为82株（81%）。与蔗糖 - 磷酸盐 - 谷氨酸缓冲液相比，从转运管中检测到的病毒分离株中有25株（24.7%）通过细胞病变效应在比蔗糖 - 磷酸盐 - 谷氨酸缓冲液早1至11天的时间被检测到。转运管还允许在环境温度下进行运输。
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