Phillips L E, Magliolo R A, Stehlik M L, Whiteman P A, Faro S, Rogers T E
J Clin Microbiol. 1985 Aug;22(2):255-8. doi: 10.1128/jcm.22.2.255-258.1985.
A total of 442 specimens from various anatomic sites was cultured for herpes simplex virus during the past 2 years. Most specimens were obtained from the respiratory tracts and cutaneous lesions of immunocompromised hosts (232 specimens) or the female genital tract (138 specimens). Two tubes containing human newborn foreskin fibroblasts and two Ortho Cultureset tubes containing Vero cells were inoculated with each specimen. The 384 inoculated specimens were stained with Cultureset peroxidase-antiperoxidase reagents within 48 h and again at 3, 4, or 5 days if initially negative. Fibroblasts were inspected for cytopathic effect for 7 days. Of these 384 specimens, Cultureset detected 57 of 62 positive specimens within 48 h; fibroblasts detected 58 positive specimens by 7 days. The calculated sensitivity and specificity for Cultureset at 48 h were 91.9 and 100%, respectively. However, when all results were considered, including those that became positive in Cultureset after 48 h, the calculated sensitivity and specificity for Cultureset were 98.8 and 100%, respectively. We conclude that Cultureset is a reliable method for detection of herpes simplex virus when two tubes are inoculated and stained as described.
在过去两年中，共对来自不同解剖部位的442份标本进行了单纯疱疹病毒培养。大多数标本取自免疫功能低下宿主的呼吸道和皮肤病变（232份标本）或女性生殖道（138份标本）。每份标本接种两管含人新生儿包皮成纤维细胞的培养管和两管含非洲绿猴肾细胞的Ortho Cultureset培养管。对接种的384份标本在48小时内用Cultureset过氧化物酶-抗过氧化物酶试剂染色，若最初结果为阴性，则在第3、4或5天再次染色。对成纤维细胞观察7天的细胞病变效应。在这384份标本中，Cultureset在48小时内检测出62份阳性标本中的57份；成纤维细胞在7天内检测出58份阳性标本。计算得出Cultureset在48小时时的敏感性和特异性分别为91.9%和100%。然而，当考虑所有结果，包括48小时后在Cultureset中呈阳性的结果时，Cultureset的计算敏感性和特异性分别为98.8%和100%。我们得出结论，按照所述方法接种和染色两管时，Cultureset是检测单纯疱疹病毒的可靠方法。
