Gehlert D R, Speth R C, Healy D P, Wamsley J K
Life Sci. 1984 Apr 16;34(16):1565-71. doi: 10.1016/0024-3205(84)90611-8.
The microscopic localization of angiotensin II receptors in the rat brainstem has been accomplished utilizing in vitro receptor autoradiographic techniques. These receptors are highly localized to discrete nuclear regions of the brainstem. Significant densities of autoradiographic grains, indicating the presence of specifically bound [125I]-angiotensin II, were observed in regions of the film corresponding to the nucleus of the solitary tract, dorsal motor nucleus of the vagus nerve, nucleus intercalatus, nucleus commissuralis, substantia gelatinosa of the trigeminal nerve and the inferior olivary nucleus. Previous immunohistochemical studies have indicated the presence of immunoreactive angiotensin II in several of these regions, especially those concerned with central cardiovascular regulatory mechanisms. These results provide additional evidence in support of the hypothesized role of angiotensin II as a central neuromodulator in the regulation of systemic blood pressure.
利用体外受体放射自显影技术已完成了血管紧张素II受体在大鼠脑干中的微观定位。这些受体高度定位于脑干的离散核区域。在与孤束核、迷走神经背运动核、中间核、连合核、三叉神经胶状质和下橄榄核相对应的胶片区域观察到大量放射自显影颗粒密度，表明存在特异性结合的[125I] -血管紧张素II。先前的免疫组织化学研究表明，在其中几个区域存在免疫反应性血管紧张素II，特别是那些与中枢心血管调节机制有关的区域。这些结果为血管紧张素II作为系统性血压调节中的中枢神经调节剂的假设作用提供了额外证据。
