Wilks C R, Jenney E W, House J A
Can J Comp Med. 1984 Apr;48(2):179-83.
This study was conducted to develop a rapid test to detect type specific antibodies to various strains of vesicular stomatitis virus. Glycoprotein was extracted from purified vesicular stomatitis virus-Indiana 3 and used as antigen in an immunoelectroosmophoresis test (counter immunoelectrophoresis) to test reactivity with homologous hyperimmune serum and antisera to vesicular stomatitis virus-New Jersey, Indiana 1 and Indiana 2. A reaction was only detected with the homologous antiserum. A simpler preparation of antigen, a detergent extract of infected cells was also evaluated and shown to have less specificity in reactions with hyperimmune sera. This infected cell extract was, however, useful in detecting homologous antibodies in convalescent sera (natural vesicular stomatitis virus-New Jersey infection) and no reactions were detected with infected cell extracts of vesicular stomatitis virus-Indiana 1, 2 and 3.
本研究旨在开发一种快速检测方法，以检测针对水疱性口炎病毒各毒株的型特异性抗体。从纯化的水疱性口炎病毒印第安纳3型中提取糖蛋白，并将其用作免疫电渗试验（对流免疫电泳）中的抗原，以检测其与同源超免疫血清以及针对水疱性口炎病毒新泽西型、印第安纳1型和印第安纳2型的抗血清的反应性。仅在同源抗血清中检测到反应。还评估了一种更简单的抗原制剂，即感染细胞的去污剂提取物，结果表明其与超免疫血清反应时特异性较低。然而，这种感染细胞提取物可用于检测恢复期血清中的同源抗体（自然感染水疱性口炎病毒新泽西型），并且未检测到与水疱性口炎病毒印第安纳1型、2型和3型的感染细胞提取物发生反应。
