Makinde A A, Majiyagbe K A
Res Vet Sci. 1982 Nov;33(3):265-9.
Sixty-one sera from animals that had contact with Dermatophilus congolensis were examined by comparing three serological methods; counterimmunoelectrophoresis, passive haemagglutination, and agar gel diffusion, and by using four different antigenic extracts of D congolensis. The counterimmunoelectrophoresis was the most satisfactory of the methods having been found to be specific and sensitive, easy to perform and suitable for screening large numbers of samples. It was also found to have a higher antibody detection rate (82.2 per cent) than the other methods thus making it suitable for seroepidemiological surveys. It was found to be capable of detecting multiple antibodies and also revealed dissimilarities among the different antigenic extracts. The cellular antigens of D congolensis were found to detect antibody in more sera than the extracellular antigen; the cell wall extract proved to be the most satisfactory of all, detecting antibody from the largest number of sera compared to the other extracts in all the three serological tests.
通过比较三种血清学方法（对流免疫电泳、被动血凝和琼脂凝胶扩散），并使用刚果嗜皮菌的四种不同抗原提取物，对61份曾接触过刚果嗜皮菌的动物血清进行了检测。对流免疫电泳是这些方法中最令人满意的，它具有特异性和敏感性，易于操作，适用于筛查大量样本。还发现它的抗体检测率（82.2%）高于其他方法，因此适用于血清流行病学调查。它能够检测多种抗体，还揭示了不同抗原提取物之间的差异。发现刚果嗜皮菌的细胞抗原比细胞外抗原能在更多血清中检测到抗体；在所有三种血清学检测中，细胞壁提取物被证明是最令人满意的，与其他提取物相比，能从最多的血清中检测到抗体。
