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一种研究病毒糖脂受体的新方法。仙台病毒与薄层层析图谱的结合。

A novel approach to the study of glycolipid receptors for viruses. Binding of Sendai virus to thin-layer chromatograms.

作者信息

Hansson G C, Karlsson K A, Larson G, Strömberg N, Thurin J, Orvell C, Norrby E

出版信息

FEBS Lett. 1984 May 7;170(1):15-8. doi: 10.1016/0014-5793(84)81359-9.

DOI:10.1016/0014-5793(84)81359-9
PMID:6327372
Abstract

A method for the binding of virus to a silica gel thin-layer chromatogram is presented. After development the chromatogram is overlayed with the 125I-labelled virus and the bound virus is autoradiographed. Alternatively, the unlabelled virus may be detected after exposure to monoclonal antibody and labelled anti-antibody. The Sendai virus strain used did not bind to brain gangliosides earlier proposed to be receptors, but bound to human erythrocyte gangliosides. This finding may be explained by the existence of Sendai virus variants with different receptor specificities.

摘要

本文介绍了一种使病毒与硅胶薄层层析板结合的方法。展开后的层析板用125I标记的病毒覆盖，然后对结合的病毒进行放射自显影。另外，未标记的病毒在与单克隆抗体及标记的抗抗体接触后也可被检测到。所用的仙台病毒株不与先前认为是受体的脑神经节苷脂结合，但能与人红细胞神经节苷脂结合。这一发现或许可通过具有不同受体特异性的仙台病毒变体的存在来解释。

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