Farace M G, Brown B A, Raschellà G, Alexander J, Gambari R, Fantoni A, Hardies S C, Hutchison C A, Edgell M H
J Biol Chem. 1984 Jun 10;259(11):7123-8.
Beta h0 and beta h1 are two beta-like globin genes in the mouse beta-globin gene cluster whose functions have not previously been established. Transcripts of both beta h0 and beta h1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that beta h1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a beta h1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of beta h1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected beta h1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The beta h1 translation product has the electrophoretic mobility expected for the z chain. We conclude that beta h1 is a fully functional gene which codes for the embryonic z chain.
βh0和βh1是小鼠β-珠蛋白基因簇中的两个类β珠蛋白基因，其功能此前尚未明确。在源自小鼠胚胎的卵黄囊红细胞以及小鼠红白血病细胞系GM979中均发现了βh0和βh1的转录本。S1核酸酶分析表明，在胚胎红细胞和GM979细胞系中，βh1是两种转录本中含量更为丰富的一种。用βh1特异性探针与10日龄、11日龄、12日龄、13日龄和14日龄小鼠胚胎的红细胞RNA杂交，结果显示βh1 mRNA水平在10日龄和11日龄时较高，随后在胎儿进一步发育过程中下降。通过酸/尿素聚丙烯酰胺凝胶电泳对杂交筛选出的βh1 mRNA的体外翻译产物进行了分析。βh1翻译产物具有z链预期的电泳迁移率。我们得出结论，βh1是一个编码胚胎z链的功能完备的基因。
