Prasad K N, Cohrs R J, Sharma O K
Department of Radiology, University of Colorado Health Sciences Center, Denver 80262.
Biochem Cell Biol. 1990 Nov;68(11):1250-5. doi: 10.1139/o90-185.
Treatment of B-16 melanoma cells in culture with d-alpha-tocopheryl succinate (vitamin E succinate) at concentrations of 11.3 and 15.1 microM inhibited growth and induced cell differentiation in culture. Vitamin E succinate treatment decreased the levels of c-myc and H-ras specific mRNAs in melanoma cells. Similar results were obtained by the vitamin retinoic acid and the nonvitamin agents R020-1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone), an inhibitor of cyclic nucleotide phosphodiesterase (0.72 mM), and sodium butyrate (1 mM), which induced differentiation and (or) inhibited growth of melanoma cells in culture. The extent of inhibition of c-myc mRNA was greater than that of H-ras mRNA. These results indicate that vitamin E succinate induced reduction of the levels of c-myc and H-ras mRNAs is related to growth inhibition of melanoma cells in culture.
在培养物中，用浓度为11.3和15.1微摩尔的琥珀酸d-α-生育酚（维生素E琥珀酸酯）处理B-16黑色素瘤细胞，可抑制其生长并诱导细胞分化。维生素E琥珀酸酯处理降低了黑色素瘤细胞中c-myc和H-ras特异性mRNA的水平。维生素维甲酸以及非维生素试剂R020-1724（4-(3-丁氧基-4-甲氧基苄基)-2-咪唑啉酮），一种环核苷酸磷酸二酯酶抑制剂（0.72毫摩尔），和丁酸钠（1毫摩尔）也得到了类似结果，它们可诱导培养的黑色素瘤细胞分化和（或）抑制其生长。c-myc mRNA的抑制程度大于H-ras mRNA。这些结果表明，维生素E琥珀酸酯诱导的c-myc和H-ras mRNA水平降低与培养的黑色素瘤细胞生长抑制有关。
