Manicardi G, Messi P, Borghi V, Bondi M
Microbiologica. 1984 Jan;7(1):1-10.
Seven strains of Streptococcus faecalis subsp. zymogenes were studied. Plasmids originally harboured by these bacteria, codifing for drug resistance, hemolysin and gelatinase activity, were transferred to a recipient strain ( JH2 -2) by conjugation. Studies on bacteria in mating showed the formation of protoplasmatic bridges between donors and recipients; these bridges should provide for the passage of genetic material. Kinetics of acquisition of the transferable resistance were carried out in liquid medium. Plasmids harboured in donor and transconjugant strains, responsable for antibiotic-resistance, for hemolysin and gelatinase activity, were analyzed later by agarose gel electrophoresis. These analysis permitted us to demonstrate the presence of many plasmids, some conjugative with a high molecular weight and others small and non-conjugative.
对7株粪肠球菌亚种发酵粪肠球菌进行了研究。这些细菌最初携带的编码耐药性、溶血素和明胶酶活性的质粒，通过接合转移到受体菌株（JH2 -2）中。对交配细菌的研究表明，供体和受体之间形成了原生质桥；这些桥应为遗传物质的传递提供通道。在液体培养基中进行了可转移抗性获得动力学研究。随后通过琼脂糖凝胶电泳分析了供体菌株和转接合子菌株中携带的负责抗生素抗性、溶血素和明胶酶活性的质粒。这些分析使我们能够证明存在许多质粒，一些是高分子量的接合型质粒，另一些是小的非接合型质粒。
