Tsichlis P N, Strauss P G, Kozak C A
Mol Cell Biol. 1984 May;4(5):997-1000. doi: 10.1128/mcb.4.5.997-1000.1984.
Two cellular DNA regions representing common domains for proviral DNA integration ( Mlvi -1 and Mlvi -2) have been identified in Moloney murine leukemia virus-induced rat thymic lymphomas. Cellular sequences which were free of repeated DNA derived from a clone that defines the Mlvi -2 integration domain (lambda Cl228 ) were found to be highly conserved in a variety of vertebrate species that we examined, including mice, hamsters, cats, and humans. In this study, we identified the chromosomal map location of the Mlvi -2 homologous sequences in mice by using hamster-mouse somatic cell hybrids. The results show that Mlvi -2 is present on mouse chromosome 15 but is unrelated to the c-myc and c-sis proto-oncogenes, which map on the same chromosome. Since aberrations on chromosome 15 have been observed reproducibly in mouse thymomas, our data suggest that Mlvi -2 may define a novel sequence involved in the induction or progression of murine thymic lymphomas.
在莫洛尼鼠白血病病毒诱导的大鼠胸腺淋巴瘤中，已鉴定出两个代表前病毒DNA整合共同结构域的细胞DNA区域（Mlvi -1和Mlvi -2）。我们发现，来自定义Mlvi -2整合结构域的克隆（λCl228）且不含重复DNA的细胞序列，在我们检测的多种脊椎动物物种（包括小鼠、仓鼠、猫和人类）中高度保守。在本研究中，我们通过使用仓鼠-小鼠体细胞杂种鉴定了小鼠中Mlvi -2同源序列的染色体图谱位置。结果表明，Mlvi -2存在于小鼠15号染色体上，但与定位在同一条染色体上的c-myc和c-sis原癌基因无关。由于在小鼠胸腺瘤中可重复性地观察到15号染色体的畸变，我们的数据表明，Mlvi -2可能定义了一个与小鼠胸腺淋巴瘤的诱导或进展相关的新序列。
