在λ::mini-Mu噬菌体中克隆的噬菌体Mu附着区域点突变的分离。
Isolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.
作者信息
Burlingame R P, Obukowicz M G, Lynn D L, Howe M M
出版信息
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6012-6. doi: 10.1073/pnas.83.16.6012.
Abstract
Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition.
摘要
在进行mutD诱变并选择以解除Mu对λ生长的特异性复制干扰后，分离出了21种λ::mini-Mu噬菌体的衍生物，这些衍生物在Mu附着区域含有点突变。DNA序列分析表明，单个左端突变体在Mu序列的第1位发生了T→C转换，而其余20个右端突变体在末端19个碱基对内含有单碱基对插入或缺失。遗传分析表明，测序揭示的右端突变对于解除λ生长的复制抑制是必要的。这些突变体的特性表明，末端2碱基对和亚末端8碱基对反向重复序列对于Mu特异性复制转座很重要。
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