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1
Isolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.在λ::mini-Mu噬菌体中克隆的噬菌体Mu附着区域点突变的分离。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6012-6. doi: 10.1073/pnas.83.16.6012.
2
Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.λplacMu：一种噬菌体λ的可转座衍生物，用于一步构建lacZ蛋白融合体。
J Bacteriol. 1984 Jun;158(3):1084-93. doi: 10.1128/jb.158.3.1084-1093.1984.
3
Bacteriophage Mu sites and functions involved in the inhibition of lambda::mini-Mu growth.与抑制λ::mini-Mu生长相关的噬菌体Mu位点及功能。
Virology. 1990 Jul;177(1):95-105. doi: 10.1016/0042-6822(90)90463-2.
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Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda.Tn5supF，一种源自Tn5的264个碱基对的转座子，用于插入诱变和对克隆在噬菌体λ中的DNA进行测序。
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5908-12. doi: 10.1073/pnas.86.15.5908.
5
[Initial characteristics and isolation of bacterial mutants with altered ability to transpose Tn9].[转座子Tn9转座能力改变的细菌突变体的初始特征及分离]
Genetika. 1980;16(6):957-66.
6
In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.通过mini-Mu转导将DNA在体内克隆到多拷贝黏粒中。
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Identification of sequences necessary for packaging DNA into lambda phage heads.鉴定将DNA包装到λ噬菌体头部所需的序列。
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Integrative recombination of bacteriophage lambda: extent of the DNA sequence involved in attachment site function.噬菌体λ的整合重组：参与附着位点功能的DNA序列范围。
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Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.噬菌体λDNA位点的转录会阻碍大肠杆菌的生长。
J Bacteriol. 1990 Feb;172(2):1030-4. doi: 10.1128/jb.172.2.1030-1034.1990.

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DNA sequence and structure requirements for cleavage of V(D)J recombination signal sequences.V(D)J重组信号序列切割的DNA序列及结构要求。
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Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage.V(D)J 重组信号切割两步过程中不同的 DNA 序列和结构要求。
EMBO J. 1996 Jun 17;15(12):3197-206.
3
Transposition of mini-Mu containing only one of the ends of bacteriophage Mu.仅包含噬菌体Mu一端的微型Mu转座。
EMBO J. 1986 Dec 20;5(13):3687-90. doi: 10.1002/j.1460-2075.1986.tb04700.x.
4
The cis-acting DNA sequences required in vivo for bacteriophage Mu helper-mediated transposition and packaging.
Arch Microbiol. 1990;154(1):67-72. doi: 10.1007/BF00249180.
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Characterization of the C operon transcript of bacteriophage Mu.噬菌体Mu C操纵子转录本的表征
J Bacteriol. 1990 Jan;172(1):361-71. doi: 10.1128/jb.172.1.361-371.1990.
6
Structural aspects of a higher order nucleoprotein complex: induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome.
EMBO J. 1991 Oct;10(10):3051-9. doi: 10.1002/j.1460-2075.1991.tb07856.x.

本文引用的文献

1
Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.用大肠杆菌噬菌体Mu d1（Apr lac）感染鼠伤寒沙门氏菌：构建pyr::lac基因融合体。
J Bacteriol. 1981 Jan;145(1):299-305. doi: 10.1128/jb.145.1.299-305.1981.
2
Transcription of bacteriophage Mu. II. Transcription of the repressor gene.噬菌体Mu的转录。II. 阻遏基因的转录。
Mol Gen Genet. 1980;179(1):185-9. doi: 10.1007/BF00268462.
3
Frameshift mutations induced by an Escherichia coli strain carrying a mutator gene, mutD5.
Mol Gen Genet. 1983;192(3):515-6. doi: 10.1007/BF00392200.
4
Essential sites at transposon Tn 10 termini.转座子Tn10末端的必需位点。
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3452-6. doi: 10.1073/pnas.81.11.3452.
5
In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.利用可转座的Mu-lac转导噬菌体一步法在体内形成编码杂交β-半乳糖苷酶蛋白的基因融合体。
Proc Natl Acad Sci U S A. 1984 Jan;81(2):535-9. doi: 10.1073/pnas.81.2.535.
6
In vitro transposition of bacteriophage Mu: a biochemical approach to a novel replication reaction.噬菌体Mu的体外转座：一种针对新型复制反应的生化方法。
Cell. 1983 Dec;35(3 Pt 2):785-94. doi: 10.1016/0092-8674(83)90111-3.
7
The terminal nucleotides of retrovirus DNA are required for integration but not virus production.逆转录病毒DNA的末端核苷酸是整合所必需的，但不是病毒产生所必需的。
Nature. 1983;306(5939):155-60. doi: 10.1038/306155a0.
8
A new insertion sequence, IS121, is found on the Mu dI1 (Ap lac) bacteriophage and the Escherichia coli K-12 chromosome.在Mu dI1（Ap lac）噬菌体和大肠杆菌K-12染色体上发现了一种新的插入序列IS121。
J Bacteriol. 1983 Nov;156(2):669-79. doi: 10.1128/jb.156.2.669-679.1983.
9
DNA sequences at the ends of transposon Tn5 required for transposition.转座所需的转座子Tn5末端的DNA序列。
Nature. 1983;304(5923):280-2. doi: 10.1038/304280a0.
10
Transposable elements in prokaryotes.原核生物中的转座元件。
Annu Rev Genet. 1981;15:341-404. doi: 10.1146/annurev.ge.15.120181.002013.

在λ::mini-Mu噬菌体中克隆的噬菌体Mu附着区域点突变的分离。

Isolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.

作者信息

Burlingame R P, Obukowicz M G, Lynn D L, Howe M M

出版信息

Proc Natl Acad Sci U S A. 1986 Aug;83(16):6012-6. doi: 10.1073/pnas.83.16.6012.

DOI:10.1073/pnas.83.16.6012
PMID:3016733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386427/
Abstract

Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition.

摘要

在进行mutD诱变并选择以解除Mu对λ生长的特异性复制干扰后，分离出了21种λ::mini-Mu噬菌体的衍生物，这些衍生物在Mu附着区域含有点突变。DNA序列分析表明，单个左端突变体在Mu序列的第1位发生了T→C转换，而其余20个右端突变体在末端19个碱基对内含有单碱基对插入或缺失。遗传分析表明，测序揭示的右端突变对于解除λ生长的复制抑制是必要的。这些突变体的特性表明，末端2碱基对和亚末端8碱基对反向重复序列对于Mu特异性复制转座很重要。