Nesland J M, Langholm R, Marton P F
Scand J Haematol. 1984 May;32(5):552-8. doi: 10.1111/j.1600-0609.1984.tb02199.x.
In epoxy resin-embedded bone marrow biopsies from 238 cases of myeloproliferative disease and preleukaemia, crystalline inclusions were found in the bone marrow cells of 24 (10.1%), most often in patients with acute myeloproliferative disease (23%). By light microscopy the crystals were easily recognized after Giemsa staining, lying within the cytoplasm of bone marrow macrophages. By electron microscopy the hexagonal crystals were encountered chiefly within secondary lysosomes of the macrophages, but also within immature myeloid cells in one of the two cases studied. In the latter a tight-fitting membrane around the crystals suggested that they were of lysosomal origin and represented a form of abnormal granulation. We suggest that the presence of crystals in myeloid cells represents a kind of abnormal granula formation in the neoplastic cells. In the macrophages the crystals lodge in secondary lysosomes, probably after phagocytosis of crystal-bearing non-viable myeloid cells. The accumulation of crystals in lysosomes indicates resistance to degradation.
在对238例骨髓增殖性疾病和白血病前期患者进行的环氧树脂包埋骨髓活检中，24例（10.1%）的骨髓细胞中发现了晶体包涵体，最常见于急性骨髓增殖性疾病患者（23%）。通过光学显微镜观察，吉姆萨染色后晶体很容易识别，位于骨髓巨噬细胞的细胞质内。通过电子显微镜观察，六角形晶体主要出现在巨噬细胞的次级溶酶体内，但在所研究的两例中的一例中，也出现在未成熟髓细胞内。在后者中，晶体周围有一层紧密贴合的膜，提示它们起源于溶酶体，代表一种异常颗粒形成形式。我们认为髓细胞中晶体的存在代表肿瘤细胞中一种异常颗粒形成。在巨噬细胞中，晶体可能在吞噬含晶体的无活力髓细胞后，沉积在次级溶酶体中。溶酶体中晶体的积累表明其对降解具有抗性。
