Kornblith P L, Szypko P E
J Neurosurg. 1978 Apr;48(4):580-6. doi: 10.3171/jns.1978.48.4.0580.
A microtiter assay was used to study the sensitivity of 24 cultured human astrocytomas to the chemotherapeutic agent 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU). The tumor cell lines testes originated from patients who were being treated with post-operative chemotherapy within the past 24 months. At a dose level comparable to the maximum in vivo therapeutic dose level, 18 of the 24 cell lines tested showed a significant response to the drug. If in vitro response can eventually be correlated with clinical response, this microtiter assay could help to form a basis for planning more specific chemotherapeutic treatment.
采用微量滴定法研究了24种培养的人星形细胞瘤对化疗药物1,3-双(2-氯乙基)-1-亚硝基脲(卡氮芥，BCNU)的敏感性。所测试的肿瘤细胞系来源于过去24个月内接受术后化疗的患者。在与最大体内治疗剂量水平相当的剂量水平下，所测试的24种细胞系中有18种对该药物表现出显著反应。如果体外反应最终能与临床反应相关联，这种微量滴定法可为规划更具特异性的化疗治疗奠定基础。
