Hand C M, Vender J R, Black P
Department of Neurosurgery, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102-1192, USA.
J Neurooncol. 1998 Jan;36(1):1-6. doi: 10.1023/a:1005894723087.
This study concerns the use of the MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] colorimetric assay to evaluate the chemosensitivity of the C6 rat glioma cell line to a panel of twelve chemotherapeutic agents. In a previous study of in vitro chemosensitivity of human glioma cell lines, the present authors found a range of sensitivities of the respective cell lines to a panel of chemotherapeutic agents [1]. We then devised an experimental strategy to begin an in vivo evaluation of the correlation between in vitro chemosensitivity and clinical response in an in vivo animal model, such as the model employing the C6 rat glioma cell line. As a step towards utilizing the C6 rat glioma in vivo model, we carried out the present study (Part 1) to determine the correspondence between chemosensitivity to human glioma cell lines and the rat C6 glioma cell line. If a correspondence were to be found, this would enable experimental use of the C6 tumor model for in vivo testing of chemotherapeutic agents. As reported in this paper (Part 1), a correspondence was found, suggesting that the C6 rat glioma represents a suitable model of human glioma for chemotherapeutic studies. This finding served as a basis for proceeding with an in vivo study of chemotherapeutic efficacy which is the subject of a companion report [2].
本研究涉及使用MTT[3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H四氮唑溴盐]比色法来评估C6大鼠胶质瘤细胞系对一组12种化疗药物的化学敏感性。在先前一项关于人类胶质瘤细胞系体外化学敏感性的研究中，作者发现各细胞系对一组化疗药物具有不同程度的敏感性[1]。随后，我们设计了一种实验策略，开始在体内动物模型（如采用C6大鼠胶质瘤细胞系的模型）中评估体外化学敏感性与临床反应之间的相关性。作为利用C6大鼠胶质瘤体内模型的第一步，我们开展了本研究（第1部分），以确定对人类胶质瘤细胞系的化学敏感性与大鼠C6胶质瘤细胞系之间的对应关系。如果能找到对应关系，这将使C6肿瘤模型可用于化疗药物的体内试验。如本文（第1部分）所报道，已发现了对应关系，表明C6大鼠胶质瘤是用于化疗研究的人类胶质瘤的合适模型。这一发现为进行化疗疗效的体内研究奠定了基础，该体内研究是一篇配套报告[2]的主题。
