Menton D N, Simmons D J, Chang S L, Orr B Y
Anat Rec. 1984 May;209(1):29-39. doi: 10.1002/ar.1092090105.
We describe the SEM appearance of the rat endosteal bone lining cell ( BLC ) population, and the sequence of morphological changes of these cells as they self-incorporate into unmineralized bone matrix (osteoid), establish intercellular connections, and construct lacunae. The osteoblast/nascent osteocyte series was progressively unsheathed by gentle digestion of the osteoid with 0.25% collagenase. The osteoblasts which leave the polygonally packed BLC compartment rapidly develop numerous complexly branched processes that contact the processes elaborated by previous generations of maturing and mature osteocytes. As osteoblasts mature and approach the mineralization front, they appear to lose processes. The mature cells begin to form osteocyte lacunae by depositing an asymmetric perimeter of woven collagen fibrils, such that as the cells roof-over, the lacunae appear as pocketlike constructions. The collagen fibrils on the perilacunar matrix are oriented in a tangential or circular pattern, while those in the more distal matrix are arranged in a parallel pattern. With the completion of a lacuna, its wall appears to mineralize quickly, for lacunae could be recognized only when they are forming.
我们描述了大鼠骨内膜骨衬里细胞（BLC）群体的扫描电镜外观，以及这些细胞自融入未矿化骨基质（类骨质）、建立细胞间连接并构建腔隙时的形态变化序列。通过用0.25%胶原酶温和消化类骨质，逐步剥离成骨细胞/新生骨细胞系列。离开多边形排列的BLC区室的成骨细胞迅速形成许多复杂分支的突起，这些突起与前几代成熟和成熟骨细胞所形成的突起相接触。当成骨细胞成熟并接近矿化前沿时，它们似乎会失去突起。成熟细胞通过沉积编织胶原纤维的不对称周边开始形成骨细胞腔隙，这样当细胞覆盖时，腔隙就呈现为袋状结构。腔隙周围基质上的胶原纤维呈切线状或圆形排列，而更远端基质中的胶原纤维则呈平行排列。随着腔隙的形成完成，其壁似乎迅速矿化，因为只有在腔隙形成时才能识别它们。
