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从由B77病毒转化的大鼠细胞中拯救出的禽肉瘤病毒获取宿主细胞遗传信息。

Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus.

作者信息

Kotler M, Salmon S, Olshevsky U

出版信息

Arch Virol. 1984;80(4):249-64. doi: 10.1007/BF01311217.

DOI:10.1007/BF01311217
PMID:6329138
Abstract

The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus ( RB77 cells) with chick cells ( FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp 85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.

摘要

将在鸡细胞中培养的禽肉瘤病毒B77的特性与通过将由B77病毒转化的大鼠细胞(RB77细胞)与鸡细胞融合获得的病毒(FB77病毒)的特性进行了比较。通过指纹技术证明了B77和FB77病毒基因组的差异。拯救出的FB77病毒含有与正常细胞基因组同源的序列。这些结果表明,拯救出的FB77病毒是B77病毒与大鼠基因组元件之间的重组体。发现来自B77和FB77病毒的RNA具有相似的分子量。B77和FB77病毒在聚丙烯酰胺凝胶中结构蛋白和大糖蛋白(gp 85)的迁移率相同,但发现FB77病毒的小糖蛋白(gp)略大于B77病毒的gp37。

相似文献

1
Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus.从由B77病毒转化的大鼠细胞中拯救出的禽肉瘤病毒获取宿主细胞遗传信息。
Arch Virol. 1984;80(4):249-64. doi: 10.1007/BF01311217.
2
Deletion mutant of the Bratislava-77 strain of Rous sarcoma virus containing a fusion of the group-specific antigen and envelope genes.劳氏肉瘤病毒布拉迪斯拉发-77株的缺失突变体,其包含群特异性抗原与包膜基因的融合体。
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Folia Biol (Praha). 1980;26(4):244-60.
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Isolates of avian sarcoma virus Bratislava 77 (B77) oncogenic for mammals do not contain an excess of transformation-defective mutants.对哺乳动物具有致癌性的禽肉瘤病毒布拉迪斯拉发77(B77)分离株不包含过量的转化缺陷型突变体。
Neoplasma. 1980;27(6):661-8.
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本文引用的文献

1
Isolation of 16L virus: a rapidly transforming sarcoma virus from an avian leukosis virus-induced sarcoma.16L病毒的分离:一种源自禽白血病病毒诱导肉瘤的快速转化肉瘤病毒。
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Generation of transforming viruses in cultures of chicken fibroblasts infected with an avian leukosis virus.在感染禽白血病病毒的鸡成纤维细胞培养物中产生转化病毒。
J Virol. 1981 Sep;39(3):920-34. doi: 10.1128/JVI.39.3.920-934.1981.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
在噬菌体T4头部组装过程中结构蛋白的切割
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The nucleotide sequence complexity of avian tumor virus RNA.禽肿瘤病毒RNA的核苷酸序列复杂性。
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Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs.基于病毒RNA分析的禽肿瘤病毒间基因交换的证据。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254-8. doi: 10.1073/pnas.71.10.4254.
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Avian tumor virus RNA: a comparison of three sarcoma viruses and their transformation-defective derivatives by oligonucleotide fingerprinting and DNA-RNA hybridization.禽肿瘤病毒RNA:通过寡核苷酸指纹图谱和DNA-RNA杂交对三种肉瘤病毒及其转化缺陷衍生物的比较
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2266-70. doi: 10.1073/pnas.70.8.2266.
8
Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information.哈维肉瘤病毒:一种带有大鼠遗传信息的第二种鼠类C型肉瘤病毒。
J Virol. 1974 Jun;13(6):1211-9. doi: 10.1128/JVI.13.6.1211-1219.1974.
9
Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species.禽白血病和肉瘤病毒RNA在甲酰胺中的凝胶电泳:与其他病毒和细胞RNA种类的比较
J Virol. 1973 Sep;12(3):594-9. doi: 10.1128/JVI.12.3.594-599.1973.
10
Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses.源自内源性和外源性禽RNA肿瘤病毒的基因重组体和杂合子。
Virology. 1973 Apr;52(2):535-52. doi: 10.1016/0042-6822(73)90349-8.