Boccara M, Pluquet N, Coll J, Rommens C, Stehelin D
J Virol. 1982 Sep;43(3):925-34. doi: 10.1128/JVI.43.3.925-934.1982.
Using biochemical methods, we have shown that a new specific sequence, v-lil, is associated with a given stock of B77 avian sarcoma virus (clone 9). We prepared a DNA complementary to v-lil sequences, using substractive hybridizations, and investigated the properties of this sequence. v-lil has a genetic complexity of ca. 2,000 nucleotides and is not present in various stocks of avian sarcoma virus, avian leukosis virus, or defective leukemia virus. v-lil is not associated with B77 avian sarcoma virus isolated from the original tumor and thus has been acquired by in vitro passage of the virus on chicken embryo fibroblasts. A search for the origin of the v-lil sequence among the DNAs of different avian species has shown that a similar sequence, c-lil, is present in normal chicken DNA (1 to 2 copies per haploid genome). c-lil is not highly conserved but is present in the DNA of all chickens from the genus Gallus. The c-lil sequence is transcribed at a low level (1 to 3 copies per cell) in normal chicken embryo fibroblasts. The biological function, if any, of v-lil or its cellular equivalent has yet to be determined.
我们运用生化方法表明，一个新的特定序列v-lil与特定株的B77禽肉瘤病毒（克隆9）相关联。我们通过扣除杂交制备了与v-lil序列互补的DNA，并研究了该序列的特性。v-lil的遗传复杂度约为2000个核苷酸，在各种禽肉瘤病毒株、禽白血病病毒株或缺陷型白血病病毒中均不存在。v-lil与从原始肿瘤分离出的B77禽肉瘤病毒不相关，因此是该病毒在鸡胚成纤维细胞上进行体外传代时获得的。在不同禽类物种的DNA中寻找v-lil序列的起源，结果表明正常鸡DNA中存在一个相似序列c-lil（单倍体基因组中每1至2个拷贝）。c-lil并非高度保守，但存在于所有原鸡属鸡的DNA中。c-lil序列在正常鸡胚成纤维细胞中以低水平转录（每个细胞1至3个拷贝）。v-lil或其细胞等价物的生物学功能（如果有的话）尚未确定。
