Speziale S C, Smith R J
Clin Immunol Immunopathol. 1985 Jul;36(1):60-9. doi: 10.1016/0090-1229(85)90039-x.
We report here that the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (FMLP), and the mitogenic phorbol ester, phorbol myristate acetate (PMA) cause a time- and concentration-dependent, selective, extracellular release of N-acetyl-beta-glucosaminidase and lysozyme from freshly isolated, adherent human peripheral blood monocytes. The inability of the protein synthesis inhibitor, cycloheximide, to influence enzyme release indicates that these enzymes are constitutive secretory products. 1-O-Hexadecyl-/octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine demonstrated moderate secretory activity, whereas pepstatin A, concanavalin A, and leukotriene B4 were essentially inactive. FMLP- and PMA-induced enzyme release were inhibited with the intracellular calcium antagonist, 8-(N,N-diethylamino)-octyl-(3,4,5-trimethoxy)benzoate hydrochloride and the anion channel blocker, 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene. These results demonstrate the capacity of soluble, surface-active stimuli to activate the human monocyte secretory process.
我们在此报告，趋化肽N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和促有丝分裂佛波酯佛波醇肉豆蔻酸酯乙酸酯（PMA）可导致从新鲜分离的贴壁人外周血单核细胞中，以时间和浓度依赖性、选择性地胞外释放N-乙酰-β-氨基葡萄糖苷酶和溶菌酶。蛋白质合成抑制剂环己酰亚胺无法影响酶的释放，这表明这些酶是组成型分泌产物。1-O-十六烷基/十八烷基-2-O-乙酰基-sn-甘油-3-磷酸胆碱表现出适度的分泌活性，而胃蛋白酶抑制剂A、伴刀豆球蛋白A和白三烯B4基本无活性。细胞内钙拮抗剂盐酸8-（N，N-二乙氨基）辛基-（3,4,5-三甲氧基）苯甲酸酯和阴离子通道阻滞剂4,4'-二异硫氰酸-2,2'-二磺酸芪可抑制FMLP和PMA诱导的酶释放。这些结果证明了可溶性表面活性刺激物激活人单核细胞分泌过程的能力。
