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小鼠免疫球蛋白轻链基因的转录起始点

Origin of transcription of a mouse immunoglobulin light chain gene.

作者信息

Bodary S, Mach B

出版信息

EMBO J. 1982;1(6):719-24. doi: 10.1002/j.1460-2075.1982.tb01236.x.

DOI:10.1002/j.1460-2075.1982.tb01236.x
PMID:6329700
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553274/
Abstract

Although the organization, the structure, and the somatic rearrangement of immunoglobulin genes have been studied in detail, there is no information regarding the precise origin of transcription of immunoglobulin genes. We have analyzed the 5'-flanking region of an expressed mouse V kappa light chain gene and the pre-mRNA transcript of that light chain gene, as it is expressed in vivo. Study of the pre-mRNA and of the DNA sequence by the procedure of "S1 mapping" establishes that the 5' end of the pre-mRNA transcript is 25-26 nucleotides upstream from the initiation codon. A CATATA sequence is found 22-23 nucleotides upstream from the origin of transcription. Although several other TATA-like sequences are found further upstream, only a single origin of transcription can be documented. From the S1 protection experiments, the 5' end of the mature mRNA was found to be the same as that of the pre-mRNA, indicating conservation of that sequence during RNA processing. Finally, a conspicuous pentanucleotide repeat CATTG - CATTG has been identified at the position of transcription initiation.

摘要

尽管免疫球蛋白基因的组织、结构和体细胞重排已得到详细研究，但关于免疫球蛋白基因转录的确切起始位置尚无相关信息。我们分析了一个已表达的小鼠Vκ轻链基因的5'侧翼区域以及该轻链基因在体内表达时的前体mRNA转录本。通过“S1作图”方法对前体mRNA和DNA序列进行研究，确定前体mRNA转录本的5'末端位于起始密码子上游25 - 26个核苷酸处。在转录起始点上游22 - 23个核苷酸处发现了一个CATATA序列。尽管在更上游还发现了其他几个类似TATA的序列，但只能证明有一个单一的转录起始点。通过S1保护实验发现，成熟mRNA的5'末端与前体mRNA的相同，这表明该序列在RNA加工过程中得以保留。最后，在转录起始位置鉴定出一个显著的五核苷酸重复序列CATTG - CATTG。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/845bc7a592cd/emboj00298-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/35cdbc2b4bfa/emboj00298-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/171a13d50241/emboj00298-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/845bc7a592cd/emboj00298-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/35cdbc2b4bfa/emboj00298-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/171a13d50241/emboj00298-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c43/553274/845bc7a592cd/emboj00298-0063-a.jpg

相似文献

1
Origin of transcription of a mouse immunoglobulin light chain gene.小鼠免疫球蛋白轻链基因的转录起始点
EMBO J. 1982;1(6):719-24. doi: 10.1002/j.1460-2075.1982.tb01236.x.
2
Transcription of mouse kappa chain genes: implications for allelic exclusion.小鼠κ链基因的转录：对等位基因排斥的影响。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1937-41. doi: 10.1073/pnas.77.4.1937.
3
RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.RNA剪接从异常重排的κ基因产生一种可变轻链。
Nature. 1980 Aug 21;286(5775):776-9. doi: 10.1038/286776a0.
4
Introns excised from immunoglobulin pre-mRNAs exist as discrete species.从免疫球蛋白前体信使核糖核酸中切除的内含子以离散的形式存在。
Mol Cell Biol. 1984 Oct;4(10):2017-22. doi: 10.1128/mcb.4.10.2017-2022.1984.
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Somatic DNA rearrangement generates functional rat immunoglobulin kappa chain genes: the J kappa gene cluster is longer in rat than in mouse.体细胞DNA重排产生功能性大鼠免疫球蛋白κ链基因：大鼠的Jκ基因簇比小鼠的更长。
Gene. 1982 May;18(2):165-74. doi: 10.1016/0378-1119(82)90114-7.
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Variable and constant regions are separated in the 10-kbase transcription unit coding for immunoglobulin kappa light chains.在编码免疫球蛋白κ轻链的10千碱基转录单元中，可变区和恒定区是分开的。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6044-8. doi: 10.1073/pnas.75.12.6044.
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Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.免疫球蛋白κ基因的正确转录需要一个包含保守序列元件的上游片段。
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The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.MPC-11细胞中特异性编码重链和轻链免疫球蛋白的信使核糖核酸的合成与加工。
Cell. 1978 Dec;15(4):1495-509. doi: 10.1016/0092-8674(78)90072-7.
9
Fine mapping of an immunoglobulin gene activator.免疫球蛋白基因激活剂的精细定位
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Immunoglobulin light chain mRNA is processed from large nuclear RNA.免疫球蛋白轻链信使核糖核酸由大核核糖核酸加工而成。
Proc Natl Acad Sci U S A. 1978 Jan;75(1):342-5. doi: 10.1073/pnas.75.1.342.

引用本文的文献

1
Mouse histocompatibility genes: structure and organisation of a Kd gene.小鼠组织相容性基因：Kd基因的结构与组织
EMBO J. 1983;2(2):245-54. doi: 10.1002/j.1460-2075.1983.tb01413.x.
2
Correct transcription of a cloned mouse immunoglobulin gene in vivo.
Proc Natl Acad Sci U S A. 1983 Jan;80(2):417-21. doi: 10.1073/pnas.80.2.417.
3
Interstrain conservation of the murine GAT-specific antibody V kappa repertoire as analyzed at the germline gene level.在种系基因水平分析的小鼠GAT特异性抗体Vκ基因库的品系间保守性。

本文引用的文献

1
A transcription map for the rabbit beta-globin gene.兔β-珠蛋白基因的转录图谱。
Cell. 1981 Feb;23(2):573-84. doi: 10.1016/0092-8674(81)90153-7.
2
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.海胆组蛋白mRNA末端位于假定调控序列下游的基因区域。
Nature. 1980 May 15;285(5761):147-51. doi: 10.1038/285147a0.
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Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
EMBO J. 1983;2(10):1771-6. doi: 10.1002/j.1460-2075.1983.tb01656.x.
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Functional modularity in the SP6 kappa promoter.SP6 κ启动子中的功能模块性
Nucleic Acids Res. 1991 Aug 25;19(16):4347-54. doi: 10.1093/nar/19.16.4347.
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
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Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.κ免疫球蛋白基因V-J重组交叉点的变异：来自一个隐蔽基因的证据。
Cell. 1980 Oct;21(3):793-9. doi: 10.1016/0092-8674(80)90442-0.
5
Functional and non-functional joining in immunoglobulin light chain genes of a mouse myeloma.小鼠骨髓瘤免疫球蛋白轻链基因中的功能性和非功能性连接
Nature. 1980 Oct 16;287(5783):603-7. doi: 10.1038/287603a0.
6
A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.一种突变的免疫球蛋白轻链是由异常的DNA和RNA剪接事件形成的。
Nature. 1980 Aug 21;286(5775):779-83. doi: 10.1038/286779a0.
7
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.从小鼠骨髓瘤肿瘤总RNA中纯化小鼠免疫球蛋白重链信使RNA。
Eur J Biochem. 1980 Jun;107(2):303-14. doi: 10.1111/j.1432-1033.1980.tb06030.x.
8
Transcription of mouse kappa chain genes: implications for allelic exclusion.小鼠κ链基因的转录：对等位基因排斥的影响。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1937-41. doi: 10.1073/pnas.77.4.1937.
9
Recombination events near the immunoglobulin Cmu gene join variable and constant region genes, switch heavy-chain expression, or inactivate the locus.免疫球蛋白Cμ基因附近的重组事件会连接可变区和恒定区基因、转换重链表达或使该基因座失活。
Biochemistry. 1981 Apr 28;20(9):2662-71. doi: 10.1021/bi00512a047.
10
Rearranged and germline immunoglobulin kappa genes: different states of DNase I sensitivity of constant kappa genes in immunocompetent and nonimmune cells.重排和种系免疫球蛋白κ基因：免疫活性细胞和非免疫细胞中恒定κ基因的不同脱氧核糖核酸酶I敏感性状态
Biochemistry. 1981 Feb 17;20(4):990-6. doi: 10.1021/bi00507a053.