小鼠骨髓瘤免疫球蛋白轻链基因中的功能性和非功能性连接

Functional and non-functional joining in immunoglobulin light chain genes of a mouse myeloma.

作者信息

Altenburger W, Steinmetz M, Zachau H G

出版信息

Nature. 1980 Oct 16;287(5783):603-7. doi: 10.1038/287603a0.

Abstract

Leader, variable (V) and joining (J) gene segments, and adjacent regions of two rearranged alleles of the same kappa-chain producing mouse myeloma, comprising approximately 3,200 base pairs, have been sequenced. Sequence comparisons are reported. V-J joining in one of the alleles leads to a reading frame with a stop codon within the J-gene segments. Allelic exclusion is apparently realized in this tumour through the formation of such a non-functional allele.

摘要

已对产生κ链的同一只小鼠骨髓瘤的两个重排等位基因的前导序列、可变（V）基因节段和连接（J）基因节段以及相邻区域进行了测序，这些区域约含3200个碱基对。报告了序列比较结果。其中一个等位基因中的V-J连接导致在J基因节段内出现带有终止密码子的阅读框。在该肿瘤中，等位基因排斥显然是通过形成这样一个无功能的等位基因来实现的。