Expression of a herpes simplex virus glycoprotein in two clonal lines of hamster cells transformed by herpes simplex virus type 2.

Expression of herpes simplex virus type 2 (HSV-2) glycoproteins on the cell surface of two clones of a hamster cell line transformed by HSV-2 was examined by immunofluorescence tests with hamster antisera prepared against three size-class glycoprotein fractions obtained from hamster embryo fibroblast cells infected with HSV-2. Enhanced expression of one of the three size-class glycoprotein fractions (molecular weight, 82,000-94,000) was found in one clone (155-4-213) after replating of the cells, and in the other (155-4-03) after actinomycin D treatment. Enhanced expression of the antigen in clone 155-4-213 was inhibited by 2-deoxy-D-glucose, but not by puromycin or protease inhibitors, antipain and p-nitrophenyl-p'-guanidinobenzoate. On the other hand, the antigen expression induced by actinomycin D in clone 155-4-03 was inhibited by these four drugs. These results indicate that the expressions of the antigen in the two clones are enhanced by different mechanisms.