Lewis J G, Kucera L S, Eberle R, Courtney R J
J Virol. 1982 Apr;42(1):275-82. doi: 10.1128/JVI.42.1.275-282.1982.
Rat embryo fibroblasts transformed by herpes simplex virus type 2 (HSV-2) were assayed for the expression of certain virus-specific glycoproteins on the surface membranes. Monospecific antisera to HSV-2-specific glycoproteins, designated gAgB, gC, and gX, were used in membrane immunofluorescence studies with HSV-2-transformed cell lines tREF-G-1, tREF-G-2, and a tumor-derived rat fibrosarcoma cells line produced in syngeneic rats inoculated with tREF-G-1 cells. Analysis of the three HSV-2-transformed cell lines showed that antisera to the gAgB and gX glycoproteins were reactive with these cells. In contrast, no significant reactivity was observed when anti-gC serum was reacted with the HSV-2-transformed cell lines. All three antiglycoprotein sera reacted positively with rat cells productively infected with HSV-2. Additionally, the HSV-2-transformed and tumor-derived cell lines showed positive internal immunofluorescence after reaction with antiserum to an early, nonstructural viral protein designated VP143 (molecular weight, 143,000). Infectivity of HSV-2 in standard plaque assays was neutralized by hyperimmune rat antisera to tREF-G-2 or rat fibrosarcoma cells and to HSV-2 virions and by sera from rats bearing the fibrosarcoma. Adsorption of rat-anti-HSV-2 serum with tREF-G-2 or rat fibrosarcoma cells reduced neutralizing activity to 10 and 12%, respectively, compared with 90% neutralization by antiserum adsorbed with nontransformed rat embryo fibroblast cells and 100% neutralization with unadsorbed antiserum. In summary, HSV-2-transformed rat cells retained and expressed genetic information necessary for the production of HSV-2 glycoproteins and a nonstructural protein after high passage in tissue culture or in the syngeneic host.
对单纯疱疹病毒2型（HSV - 2）转化的大鼠胚胎成纤维细胞进行检测，以确定某些病毒特异性糖蛋白在细胞膜表面的表达情况。使用针对HSV - 2特异性糖蛋白（分别命名为gAgB、gC和gX）的单特异性抗血清，对HSV - 2转化的细胞系tREF - G - 1、tREF - G - 2以及在接种了tREF - G - 1细胞的同基因大鼠中产生的肿瘤源性大鼠纤维肉瘤细胞系进行膜免疫荧光研究。对这三种HSV - 2转化的细胞系分析表明，针对gAgB和gX糖蛋白的抗血清与这些细胞有反应。相比之下，当抗gC血清与HSV - 2转化的细胞系反应时，未观察到明显反应。所有三种抗糖蛋白血清与被HSV - 2有效感染的大鼠细胞均呈阳性反应。此外，在与针对一种早期非结构病毒蛋白VP143（分子量143,000）的抗血清反应后，HSV - 2转化的细胞系和肿瘤源性细胞系显示出阳性的内部免疫荧光。在标准空斑试验中，HSV - 2的感染性被针对tREF - G - 2或大鼠纤维肉瘤细胞以及HSV - 2病毒粒子的超免疫大鼠抗血清中和，也被患有纤维肉瘤的大鼠的血清中和。用tREF - G - 2或大鼠纤维肉瘤细胞吸附大鼠抗HSV - 2血清后，中和活性分别降至10%和12%，而用未转化的大鼠胚胎成纤维细胞吸附的抗血清中和率为90%，未吸附的抗血清中和率为100%。总之，HSV - 2转化的大鼠细胞在组织培养或同基因宿主体内经过多次传代后，仍保留并表达产生HSV - 2糖蛋白和一种非结构蛋白所需的遗传信息。
