Tsuji T, Takahashi K, Naito K, Nagashima H
Gastroenterol Jpn. 1984 Apr;19(2):116-20. doi: 10.1007/BF02806933.
A simplified single radial immunodiffusion (SRID) method to detect HBsAg particles albumin receptor activity in regard to polymerized human serum albumin (pHSA) (binding-activity of pHSA = pHSA-BA or albumin receptor activity) was developed and its significance in HBeAg-positive hepatitis B patients after a one year follow-up study was discussed. The pHSA-BA determined by the simplified SRID method was classified into 5 grades; +, +/- and -, and the results of this grading of test sera agreed well with those obtained by the ELISA quantitative assay. According to the results of one year follow-up study in sero-changes of HBeAg in 35 HBeAg-positive cases, 7 cases with pHSA-BA did not show HBeAg sero-change, but of 16 cases with pHSA-BA and 12 cases with (+) pHSA-BA 3 (18.8%) and 7 (58.3%) cases showed HBeAg sero-changes, respectively. These results suggest that the present simplified SRID detection of albumin receptor on HBsAg particles might be a useful method for the laboratory investigation of hepatitis B patients.
开发了一种简化的单放射免疫扩散（SRID）方法，用于检测HBsAg颗粒对聚合人血清白蛋白（pHSA）的白蛋白受体活性（pHSA的结合活性=pHSA-BA或白蛋白受体活性），并讨论了其在HBeAg阳性乙型肝炎患者一年随访研究中的意义。通过简化的SRID方法测定的pHSA-BA分为5个等级：+、+/-和-，测试血清的该分级结果与ELISA定量测定结果非常一致。根据对35例HBeAg阳性病例HBeAg血清学变化的一年随访研究结果，7例pHSA-BA病例未出现HBeAg血清学变化，但16例pHSA-BA病例和12例（+）pHSA-BA病例中分别有3例（18.8%）和7例（58.3%）出现HBeAg血清学变化。这些结果表明，目前简化的SRID检测HBsAg颗粒上的白蛋白受体可能是乙型肝炎患者实验室研究的一种有用方法。
