Gillin F D, Reiner D S, McCann P P
J Protozool. 1984 Feb;31(1):161-3. doi: 10.1111/j.1550-7408.1984.tb04308.x.
Difluoromethylornithine (DFMO) is a specific and irreversible inhibitor of ornithine decarboxylase, an enzyme which catalyzes the first step in the biosynthetic pathway of the polyamines. We tested the effect of DFMO on the growth of Giardia lamblia, Entamoeba histolytica, and Trichomonas vaginalis. Growth of G. lamblia was inhibited by DFMO at concentrations of greater than or equal to 1.25 mM. Culture doubling time increased with increasing DFMO concentration. Growth inhibition was reversed if spermidine was added within 53 h of addition of DFMO; no growth was observed if spermidine was added later, indicating eventual parasite death. The growth of E. histolytica and T. vaginalis, two unrelated mucosal-dwelling parasites of humans, was not inhibited by 20 mM DFMO. These studies indicate that polyamine biosynthesis from ornithine is required for growth of G. lamblia.
二氟甲基鸟氨酸（DFMO）是鸟氨酸脱羧酶的一种特异性不可逆抑制剂，鸟氨酸脱羧酶是催化多胺生物合成途径第一步的酶。我们测试了DFMO对蓝氏贾第鞭毛虫、溶组织内阿米巴和阴道毛滴虫生长的影响。当DFMO浓度大于或等于1.25 mM时，蓝氏贾第鞭毛虫的生长受到抑制。培养物的倍增时间随DFMO浓度的增加而增加。如果在添加DFMO后的53小时内添加亚精胺，生长抑制作用可被逆转；如果稍后添加亚精胺，则未观察到生长，表明寄生虫最终死亡。20 mM的DFMO对溶组织内阿米巴和阴道毛滴虫这两种与人类无关的栖居在黏膜的寄生虫的生长没有抑制作用。这些研究表明，鸟氨酸合成多胺是蓝氏贾第鞭毛虫生长所必需的。
