DNA甲基化的生物学作用：与大肠杆菌DNA中GATC位点低甲基化相关的序列特异性单链断裂
Biological role of DNA methylation: sequence-specific single-strand breaks associated with hypomethylation of GATC sites in Escherichia coli DNA.
作者信息
Szyf M, Meisels E, Razin A
出版信息
J Bacteriol. 1986 Dec;168(3):1487-90. doi: 10.1128/jb.168.3.1487-1490.1986.
Abstract
The effect of methylation of GATC sites in Escherichia coli DNA on the formation of single-strand breaks was studied with dam+, dam mutant, and Dam-overproducer strains. Single-strand breaks have been observed in dam mutant cells predominantly at TpT and, to a lesser extent, at CpC. In dam mutant cells harboring pTP166 (a plasmid containing the dam gene), no such nicks were observed.
摘要
利用dam+菌株、dam突变体菌株和Dam过表达菌株，研究了大肠杆菌DNA中GATC位点甲基化对单链断裂形成的影响。在dam突变体细胞中，主要在TpT处观察到单链断裂，在CpC处观察到的程度较小。在携带pTP166（一种含有dam基因的质粒）的dam突变体细胞中，未观察到此类切口。
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