Jensen E O, Marcker K A, Villadsen I S
EMBO J. 1986 May;5(5):843-7. doi: 10.1002/j.1460-2075.1986.tb04293.x.
The TM1 yeast mutant was transformed with a 2 micron-derived plasmid (YEp24) which carries a chimaeric gene containing the Escherichia coli chloramphenicol acetyl transferase (CAT) gene fused to the 5'- and 3'-flanking regions of the soybean leghemoglobin (Lb) c3 gene. Expression of the chimaeric CAT gene is controlled specifically by heme at a post-transcriptional level, most likely by regulating the efficiencies of translation. Expression of another chimaeric gene consisting of the neomycin phosphotransferase (NPTII) gene fused to only the 5'-flanking region of the Lbc3 gene is regulated by heme in a similar way. Thus, in yeast, heme modulates the translation of the chimaeric mRNAs through interactions with the 5' Lbc3 non-coding region.
用一个源自2微米质粒（YEp24）转化TM1酵母突变体，该质粒携带一个嵌合基因，此嵌合基因包含与大豆豆血红蛋白（Lb）c3基因的5'和3'侧翼区域融合的大肠杆菌氯霉素乙酰转移酶（CAT）基因。嵌合CAT基因的表达在转录后水平上由血红素特异性控制，最有可能是通过调节翻译效率来实现。另一个由新霉素磷酸转移酶（NPTII）基因与仅Lbc3基因的5'侧翼区域融合而成的嵌合基因的表达也以类似方式受血红素调节。因此，在酵母中，血红素通过与Lbc3基因5'非编码区域相互作用来调节嵌合mRNA的翻译。
