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一种景天酸代谢植物磷酸烯醇式丙酮酸羧化酶-核酮糖-1,5-二磷酸羧化酶/加氧酶嵌合基因的光调节：来自C3植物的光调节控制序列在景天酸代谢植物细胞中发挥作用。

Light regulation of a SSRubisco-nos chimaeric gene: photoregulatory control sequences from a C3 plant function in cells of a CAM plant.

作者信息

Shaw C H, Sanders D M, Bates M R, Shaw C H

出版信息

Nucleic Acids Res. 1986 Aug 26;14(16):6603-12. doi: 10.1093/nar/14.16.6603.

DOI:10.1093/nar/14.16.6603
PMID:3018675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311667/
Abstract

A SSRubisco-nos chimaeric gene has been constructed, in an oncogenic Ti-plasmid vector. A 900bp soybean SSRubisco upstream fragment, carrying CAAT and TATA boxes and transcription initiation point, was fused to the nos coding region, the fusion site being within the 5'-untranslated region. When this chimaeric construct was transferred to Kalanchoe cells, nos expression was shown to be light-regulated. Thus DNA sequences responsible for light-dark control of gene expression are wholly or partly contained within the 900bp soybean SSRubisco upstream region. Moreover, this is the first demonstration that photoregulatory elements in a gene derived from a C3 plant, function in cells of a plant exhibiting the CAM trait.

摘要

一个SSRubisco-nos嵌合基因已在致癌Ti质粒载体中构建完成。一个携带CAAT盒、TATA盒和转录起始点的900bp大豆SSRubisco上游片段与nos编码区融合，融合位点位于5'非翻译区内。当这个嵌合构建体被转移到长寿花细胞中时，发现nos的表达受光调节。因此，负责基因表达明暗调控的DNA序列全部或部分包含在900bp大豆SSRubisco上游区域内。此外，这是首次证明来自C3植物的基因中的光调节元件在具有景天酸代谢（CAM）特性的植物细胞中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5cf/311667/ddf8b4209c3a/nar00285-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5cf/311667/ddf8b4209c3a/nar00285-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5cf/311667/ddf8b4209c3a/nar00285-0267-a.jpg

相似文献

1
Light regulation of a SSRubisco-nos chimaeric gene: photoregulatory control sequences from a C3 plant function in cells of a CAM plant.一种景天酸代谢植物磷酸烯醇式丙酮酸羧化酶-核酮糖-1,5-二磷酸羧化酶/加氧酶嵌合基因的光调节：来自C3植物的光调节控制序列在景天酸代谢植物细胞中发挥作用。
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2
Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector.使用Ti质粒载体将嵌合基因导入烟草后，该基因的光诱导性及叶绿体相关表达
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引用本文的文献

1
5' proximal sequences of a soybean ribulose-1,5-bisphosphate carboxylase small subunit gene direct light and phytochrome controlled transcription.大豆核酮糖-1,5-二磷酸羧化酶小亚基基因的5'近端序列指导光和光敏色素控制的转录。
Nucleic Acids Res. 1987 Aug 25;15(16):6501-14. doi: 10.1093/nar/15.16.6501.
2
A potential role for RNA turnover in the light regulation of plant gene expression: ribulose-1,5-bisphosphate carboxylase small subunit in soybean.RNA周转在植物基因表达光调控中的潜在作用：大豆中的1,5-二磷酸核酮糖羧化酶小亚基
Nucleic Acids Res. 1990 Jun 11;18(11):3377-85. doi: 10.1093/nar/18.11.3377.

本文引用的文献

1
Demonstration of transcriptional regulation of specific genes by phytochrome action.展示了光敏色素作用对特定基因的转录调控。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1112-6. doi: 10.1073/pnas.81.4.1112.
2
Photoregulated expression of a pea rbcS gene in leaves of transgenic plants.光调控豌豆 rbcS 基因在转基因植物叶片中的表达。
EMBO J. 1985 Dec 1;4(12):3063-8. doi: 10.1002/j.1460-2075.1985.tb04046.x.
3
Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5'-flanking sequence of a pea chlorophyll a/b-binding protein gene.
在豌豆叶绿素 a/b 结合蛋白基因 5'侧翼序列控制下，嵌合基因的光诱导和组织特异性表达。
EMBO J. 1985 Nov;4(11):2723-9. doi: 10.1002/j.1460-2075.1985.tb03995.x.
4
High level expression of introduced chimaeric genes in regenerated transformed plants.导入的嵌合基因在再生转化植株中的高水平表达。
EMBO J. 1985 Oct;4(10):2411-8. doi: 10.1002/j.1460-2075.1985.tb03949.x.
5
The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts.利用核编码序列指导外源蛋白的光调控合成并转运至植物叶绿体。
EMBO J. 1985 Jan;4(1):25-32. doi: 10.1002/j.1460-2075.1985.tb02312.x.
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Nopaline synthase: transcript mapping and DNA sequence.胭脂碱合成酶：转录图谱与DNA序列
J Mol Appl Genet. 1982;1(6):561-73.
7
The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean.大豆中编码1,5-二磷酸核酮糖羧化酶小亚基的多基因家族一个成员的核苷酸序列、表达及进化
J Mol Appl Genet. 1982;1(6):483-98.
8
Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.根癌土壤杆菌Ti质粒的位点特异性诱变及基因向植物细胞的转移。
J Mol Appl Genet. 1981;1(2):149-64.
9
Nuclear and polysomal transcripts of T-DNA in octopine crown gall suspension and callus cultures.章鱼碱型冠瘿悬浮培养物和愈伤组织培养物中T-DNA的核转录本和多核糖体转录本。
Mol Gen Genet. 1981;182(2):255-62. doi: 10.1007/BF00269667.
10
Light-regulated expression of a pea ribulose-1,5-bisphosphate carboxylase small subunit gene in transformed plant cells.豌豆核酮糖-1,5-二磷酸羧化酶小亚基基因在转化植物细胞中的光调控表达。
Science. 1984 May 25;224(4651):838-43. doi: 10.1126/science.6719112.