玉米中1,5-二磷酸核酮糖羧化酶大亚基的单个基因产生两种受不同调控的mRNA。
Single gene for the large subunit of ribulosebisphosphate carboxylase in maize yields two differentially regulated mRNAs.
作者信息
Crossland L D, Rodermel S R, Bogorad L
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4060-4. doi: 10.1073/pnas.81.13.4060.
A second mRNA coding for the large subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxylase (dimerizing), EC 4.1.1.39] has been found in the plastids of maize leaves. The 5' terminus of this mRNA was shown by S1 nuclease analysis to be 238 nucleotides upstream of the previously described large subunit mRNA [McIntosh, L., Poulsen, C. & Bogorad, L. (1980) Nature (London) 288, 556-560]. The same two mRNAs were produced in a homologous in vitro transcription system using cloned plastid DNA as a template. The ratio of the two mRNAs changes during light-induced plastid development and can be altered in the in vitro system by manipulation of transcription conditions.
在玉米叶片的质体中发现了另一种编码1,5-二磷酸核酮糖羧化酶[3-磷酸-D-甘油酸羧化酶（二聚化），EC 4.1.1.39]大亚基的信使核糖核酸。通过S1核酸酶分析表明，这种信使核糖核酸的5'末端位于先前描述的大亚基信使核糖核酸的上游238个核苷酸处[麦金托什，L.，保尔森，C.和博戈拉德，L.（1980年）《自然》（伦敦）288, 556 - 560]。使用克隆的质体DNA作为模板，在同源体外转录系统中产生了相同的两种信使核糖核酸。在光诱导的质体发育过程中，这两种信使核糖核酸的比例会发生变化，并且在体外系统中可以通过操纵转录条件来改变。
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