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1,5-二磷酸核酮糖羧化酶/加氧酶大亚基与偶联因子β亚基基因之间的顺反子间区域序列

Sequence of the intercistronic region between the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit and coupling factor beta subunit gene.

作者信息

Shinozaki K, Sugiura M

出版信息

Nucleic Acids Res. 1982 Aug 25;10(16):4923-34. doi: 10.1093/nar/10.16.4923.

DOI:10.1093/nar/10.16.4923
PMID:6290996
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320842/
Abstract

The nucleotide sequence of the region between the genes for the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase and the beta subunit of coupling factor from tobacco chloroplast DNA has been determined. The 5' ends of their mRNAs have been located on the DNA sequence using s1 nuclease mapping. The promoter sequences of these chloroplast genes have features in common with those of prokaryotes. E. coli RNA polymerase can recognize these sequences and start transcription at authentic initiation sites.

摘要

已确定烟草叶绿体DNA中1,5 - 二磷酸核酮糖羧化酶/加氧酶大亚基基因与偶联因子β亚基基因之间区域的核苷酸序列。利用S1核酸酶图谱分析已在DNA序列上定位了它们mRNA的5'末端。这些叶绿体基因的启动子序列具有与原核生物启动子序列相同的特征。大肠杆菌RNA聚合酶能够识别这些序列并在真实的起始位点起始转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/e0be518ba6ec/nar00385-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/5a692df46667/nar00385-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/af61edc20769/nar00385-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/7866d2ae7ef6/nar00385-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/e0be518ba6ec/nar00385-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/5a692df46667/nar00385-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/af61edc20769/nar00385-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/7866d2ae7ef6/nar00385-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eeaa/320842/e0be518ba6ec/nar00385-0108-a.jpg

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本文引用的文献

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Biosynthesis and assembly of the proton-translocating adenosine triphosphatase complex from chloroplasts.
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